大气细颗粒物PM2．5致Wistar大鼠肺部病变的研究

目的探讨大气细颗粒物（particulate matter,PM）PM25致肺部病变机制。方法采用实时PM25采集器进行PM25收集，Wistar大鼠饲养于PM25的全身暴露染毒系统，Wistar大鼠每日染毒4小时，共染毒9月，设立实验组及对照组；观察肺组织大体结构及HE染色观察；流式细胞术检~NWistar大鼠T细胞免疫功能、肺组织细胞凋亡、细胞周期及DNA指数；ELISA实验检测wistar大鼠血清及肺灌洗液中CEA、CAl2572．SccAg表达水平。结果实验组肺组织以支气管向外周辐射状颜色发暗、偏黑，多处可见白色斑点和泡状凸起，边缘不规则白色隆起；HE染色结果，实验组肺组织纤维化，肺实...

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Published in肿瘤防治研究 Vol. 44; no. 10; pp. 652 - 658
Main Author 巨英超 刘亮 贺宇彤 单保恩
Format Journal Article
LanguageChinese
Published 2017
Subjects
DNA指数
细胞增殖
细颗粒物
肺病变
雾霾
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ISSN1000-8578

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Summary:目的探讨大气细颗粒物（particulate matter,PM）PM25致肺部病变机制。方法采用实时PM25采集器进行PM25收集，Wistar大鼠饲养于PM25的全身暴露染毒系统，Wistar大鼠每日染毒4小时，共染毒9月，设立实验组及对照组；观察肺组织大体结构及HE染色观察；流式细胞术检~NWistar大鼠T细胞免疫功能、肺组织细胞凋亡、细胞周期及DNA指数；ELISA实验检测wistar大鼠血清及肺灌洗液中CEA、CAl2572．SccAg表达水平。结果实验组肺组织以支气管向外周辐射状颜色发暗、偏黑，多处可见白色斑点和泡状凸起，边缘不规则白色隆起；HE染色结果，实验组肺组织纤维化，肺实变严重，肺泡充血，见大量炎性反应增生；实验组CD4／CD8比值显著高于对照组（P〈0．05），血清及肺灌洗液中CAl25的表达显著高于对照组（P〈0．05），实验组肺组织细胞凋亡率显著低于对照组，而DNA指数显著增高（均P〈0．05）。结论长期接触PM2，会造成肺组织出现不同程度结节，肺组织出现大范围炎性反应病变。
Bibliography:42-1241/R
JU Yingchao1, LIU Liang2, HE Yutong2, SHAN Baoen2,3 1. Experimental Animal Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China; 2. Tumor Institute, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China; 3. Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
Objective To investigate the mechanism of lung lesions caused by fine particulate matter PM2.5. Methods Real-time PM2.5 collector was used to collect PM2.5. Wistar rats were fed on the systemic exposure system of PM2.5. Wistar rats were exposed to 4 hours daily for 9 months. PM2.5 experimental group and control group were set up. General structure of lung tissue and HE staining result were observed. T cell immune function, lung cell apoptosis, cell cycle and DNA index in Wistar rats were detected by flow cytometry. The expression levels of CEA, CA125 and SccAg in serum and lung lavage fluid of Wistar rats were detected by ELISA. Results Lung tissues of ex
ISSN:1000-8578