江苏H9N2亚型禽流感病毒HA和NA的遗传进化分析
为了解江苏H9N2亚型禽流感病毒（AIV）的变异情况,采用RT-PCR技术对10株从江苏不同地区分离的H9N2亚型禽流感病毒的HA和NA基因进行了扩增、克隆、测序和遗传进化分析。结果显示：10株H9N2病毒间HA基因核苷酸和氨基酸的同源性分别是94.3%-99.0%、93.8%-99.6%,NA基因核苷酸和氨基酸的同源性分别是94.9%-99.9%、95.2%-100.0%,它们均属于欧亚分支中的A/Duck/Hong Kong/Y280/97亚群;10株病毒的HA裂解位点均为RSSR-GLF,受体结合位点第198位有8株为苏氨酸,2株为丙氨酸,第191位有1株为组氨酸;10株病毒NA基因在6...
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| Published in | 江西农业学报 Vol. 29; no. 7; pp. 104 - 109 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
2017
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| Subjects | |
| Online Access | Get full text |
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| Summary: | 为了解江苏H9N2亚型禽流感病毒（AIV）的变异情况,采用RT-PCR技术对10株从江苏不同地区分离的H9N2亚型禽流感病毒的HA和NA基因进行了扩增、克隆、测序和遗传进化分析。结果显示：10株H9N2病毒间HA基因核苷酸和氨基酸的同源性分别是94.3%-99.0%、93.8%-99.6%,NA基因核苷酸和氨基酸的同源性分别是94.9%-99.9%、95.2%-100.0%,它们均属于欧亚分支中的A/Duck/Hong Kong/Y280/97亚群;10株病毒的HA裂解位点均为RSSR-GLF,受体结合位点第198位有8株为苏氨酸,2株为丙氨酸,第191位有1株为组氨酸;10株病毒NA基因在62-64位均存在缺失的现象,从而导致61位的糖基化位点缺失,NA基因第402位均出现了由天冬酰胺（N）到天冬氨酸（D）的变异,8株病毒只具有6个糖基化位点。 |
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| Bibliography: | 36-1124/S Jiangsu; H9N2 subtype; Avian influenza virus; HA; NA; Genetic evolution In order to understand the variation situation of H9N2-subtype avian influenza virus（ AIV） in Jiangsu area,we cloned the HA gene and NA gene of 10 H9N2-subtype AIV strains isolated from different regions of Jiangsu by RT-PCR amplification,and carried out the genetic evolution analysis of the HA and NA sequences. The results showed that the homology of nucleotide and amino acid of HA gene among 10 strains was 94.3% - 99.0% and 93.8 - 99.6%,respectively; the homology of nucleotide and amino acid of NA gene among 10 strains was 94.9% - 99.9% and 95.2% - 100.0%,respectively; ten strains all belonged to A/Duck/Hong Kong/Y280/97 subgroup in Eurasian clade; the HA cleavage sites of all 10 isolates were RSSR-GLF; at the198 th receptor binding site,eight of ten strains had threonine,and two of ten strains had alanine; at the 191 st receptor binding site,one of ten strains had histidine; the deletion of the 62 - 64 th sites in NA gene of al |
| ISSN: | 1001-8581 |