Sulfated polysaccharides with antioxidant and anticoagulant activity from the sea cucumber Holothuriafuscogliva

• Published in: 中国海洋湖沼学报：英文版 Vol. 35; no. 4; pp. 763 - 769
• Main Author: 李荣锋 于华华 岳洋 刘松 邢荣娥 陈晓琳 李鹏程
• Format: Journal Article
• Language: English
• Published: 2017
• Subjects: 凝血酶原时间; 抗凝血活性; 抗氧化活性; 活化部分凝血活酶时间; 海参; 生物活性成分; 硫酸多糖; 超氧阴离子自由基
• ISSN: 0254-4059; 1993-5005

Sea cucumber is a traditional nutritional food and medicinal resource with many bioactive components in China. Holothuriafuscogliva is a big sea cucumber with a rich of bioactive polysaccharides. To investigate the bioactivities of the polysaccharides from sea cucumber H. fuscogliva, we prepared the sulfated polysaccharides （HfP） from sea cucumber H. fuscogliva using a protease hydrolysis method. Antioxidant activities of HfP were investigated, including hydroxyl radical scavenging activity and superoxide radical scavenging activity. And, the anticoagulant activities of HfP were studied, including the activated partial thromboplastin time （APTT）, prothrombin time （PT） and thrombin time （TT）. The average molecular weight was 1 867.1 Da, with a sulfate content of 20.7%. In addition, the molar ratio of monosaccharide composition of HfP was Man： Rha： Glc A： Glc： Gal： Xyl： Fuc=0.083 6： 0.437： 0.134： 0： 1.182： 0.748： 1. It had a strong antioxidant activity, the hydroxyl and superoxide radical scavenging activity ECs0 of HfP was 3.74 and 0.037 mg/mL, respectively. It also showed a good anticoagulant activity in our study. The APTT of HfP was much higher than that of heparin sodium, and the PT and TT of HfP was close to that ofheparin sodium at a low concentration. Therefore, HfP shows a good antioxidant and anticoagulant activity and it may become a potential candidate of the natural antioxidant and anticoagulant and will have a good application future in health product or medicine industry.