基于近红外光谱法的党参醇沉上清液中多种成分的同时定量

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Bibliographic Details
Published in浙江大学学报：B卷英文版 Vol. 18; no. 5; pp. 383 - 392
Main Author Yu LUO Wen-long LI Wen-hua HUANG Xue-hua LIU Yan-gang SONG Hai-bin QU
Format Journal Article
LanguageEnglish
Published 2017
Subjects
党参
多成分定量
实时放行检测
近红外光谱法
醇沉
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ISSN1673-1581
1862-1783

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Bibliography:Near infrared spectroscopy; Codonopsis Radix; Alcohol precipitation; Real-time release testing; Multicomponents quantification
33-1356/Q
A near infrared spectroscopy（NIRS） approach was established for quality control of the alcohol precipitation liquid in the manufacture of Codonopsis Radix. By applying NIRS with multivariate analysis, it was possible to build variation into the calibration sample set, and the Plackett-Burman design, Box-Behnken design, and a concentrating-diluting method were used to obtain the sample set covered with sufficient fluctuation of process parameters and extended concentration information. NIR data were calibrated to predict the four quality indicators using partial least squares regression（PLSR）. In the four calibration models, the root mean squares errors of prediction（RMSEPs） were 1.22 μg/ml, 10.5 μg/ml, 1.43 μg/ml, and 0.433% for lobetyolin, total flavonoids, pigments, and total solid contents, respectively. The results indicated that multi-components quantification of the alcohol precipitation liquid of Codonopsis Radix could be achieved with an NIRS-based method, which offers a useful tool for real-time release testing（RTRT） of intermediates in the manufacture of Codonopsis Radix.
ISSN:1673-1581
1862-1783