Baicalin extracted from Huangqin（Radix Scutellariae Baicalensis） induces apoptosis in gastric cancer cells by regulating B cell lymphoma（Bcl-2）/Bcl-2-associated X protein and activating caspase-3 and caspase-9

OBJECTIVE：To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action.METHODS：Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human...

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Published in中医杂志：英文版 Vol. 37; no. 2; pp. 229 - 235
Main Author Wang Hongwei Li Hailong Chen Fengqin Luo Jun Gu Jing Wang Huping Wu Hongyan Xu Yan
Format Journal Article
LanguageEnglish
Published 2017
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Summary:OBJECTIVE：To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action.METHODS：Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 cells.Real time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of baicalin.RESULTS：In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 μmol/L baicalin for 48 h, a 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent manner.Moreover, baicalin up-regulated the expression of caspase-3, caspase-9, and B cell lymphoma（Bcl-2）-associated X protein and down-regulated the expression of Bcl-2 at both the m RNA and protein level.CONCLUSION：Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells.
Bibliography:Baicalin; Stomach neoplasms; Apoptosis Lymphoma; B-Cell; Bcl-2-associated X protein; Caspases; effector
11-2167/R
OBJECTIVE：To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of action.METHODS：Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 cells.Real time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of baicalin.RESULTS：In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 μmol/L baicalin for 48 h, a 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent manner.Moreover, baicalin up-regulated the expression of caspase-3, caspase-9, and B cell lymphoma（Bcl-2）-associated X protein and down-regulated the expression of Bcl-2 at both the m RNA and protein level.CONCLUSION：Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells.
ISSN:0255-2922