Synthesis of a Heptapeptide and Its Application in the Detection of Mercury（Ⅱ） Ion
A new heptapeptide, Cys-Pro-Gly-Cys-Pro-Gly-Cys-CONH2（P7）, was prepared via Fmoc solid-phasepepfide synthesis, purified by high performance liquid chromatography（HPLC） and characterized by mass spectro-metry（MS）. The interactions of P7 with metal ions were also investigated by spectroscopy and the r...
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| Published in | 高等学校化学研究：英文版 Vol. 33; no. 2; pp. 155 - 159 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
2017
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| Subjects | |
| Online Access | Get full text |
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| Abstract | A new heptapeptide, Cys-Pro-Gly-Cys-Pro-Gly-Cys-CONH2（P7）, was prepared via Fmoc solid-phasepepfide synthesis, purified by high performance liquid chromatography（HPLC） and characterized by mass spectro-metry（MS）. The interactions of P7 with metal ions were also investigated by spectroscopy and the results show thatP7 has highly selective binding affinity to Hg（Ⅱ）. Based on this specific character, a simple, rapid and sensitivedetection method for Hg（Ⅱ） was developed. When the mixture solution of P7 and Hg（Ⅱ） was added to colloidal goldnanoparticles（GNP）, the color of GNP remained unchanged. On the contrary, the color of GNP changed from red toblue when the mixture solutions of P7 and other metal ions were added. |
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| AbstractList | A new heptapeptide, Cys-Pro-Gly-Cys-Pro-Gly-Cys-CONH2（P7）, was prepared via Fmoc solid-phasepepfide synthesis, purified by high performance liquid chromatography（HPLC） and characterized by mass spectro-metry（MS）. The interactions of P7 with metal ions were also investigated by spectroscopy and the results show thatP7 has highly selective binding affinity to Hg（Ⅱ）. Based on this specific character, a simple, rapid and sensitivedetection method for Hg（Ⅱ） was developed. When the mixture solution of P7 and Hg（Ⅱ） was added to colloidal goldnanoparticles（GNP）, the color of GNP remained unchanged. On the contrary, the color of GNP changed from red toblue when the mixture solutions of P7 and other metal ions were added. |
| Author | FENG Huiyun GAO Lei YE Xinhui WANG Lei XUE Zechun KONG Jinming LI Lianzhi |
| AuthorAffiliation | School of Chemistry and Chemical Engineering, Liaocheng University, Liaoeheng 252059, P. R. China Zhong Yuan Academy of Biological Medicine, Liaocheng People＇s Hospital, Liaoeheng 252000, P.. R. China School of Environmental and Biological Engineering, Nanj＇ing University of Science and Technology, Nanjing 210094, P. R. China College of Innovation and Entrepreneurship, Liaocheng University, Liaocheng 252059, R R. China |
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| SubjectTerms | Absorption detection Gold Hg^2 nanoparticle Peptide spectrum |
| Title | Synthesis of a Heptapeptide and Its Application in the Detection of Mercury（Ⅱ） Ion |
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