Synthesis of a Heptapeptide and Its Application in the Detection of Mercury（Ⅱ） Ion

A new heptapeptide, Cys-Pro-Gly-Cys-Pro-Gly-Cys-CONH2（P7）, was prepared via Fmoc solid-phasepepfide synthesis, purified by high performance liquid chromatography（HPLC） and characterized by mass spectro-metry（MS）. The interactions of P7 with metal ions were also investigated by spectroscopy and the r...

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Published in高等学校化学研究：英文版 Vol. 33; no. 2; pp. 155 - 159
Main Author FENG Huiyun GAO Lei YE Xinhui WANG Lei XUE Zechun KONG Jinming LI Lianzhi
Format Journal Article
LanguageEnglish
Published 2017
Subjects
Absorption
detection
Gold
Hg^2
nanoparticle
Peptide
spectrum
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Summary:A new heptapeptide, Cys-Pro-Gly-Cys-Pro-Gly-Cys-CONH2（P7）, was prepared via Fmoc solid-phasepepfide synthesis, purified by high performance liquid chromatography（HPLC） and characterized by mass spectro-metry（MS）. The interactions of P7 with metal ions were also investigated by spectroscopy and the results show thatP7 has highly selective binding affinity to Hg（Ⅱ）. Based on this specific character, a simple, rapid and sensitivedetection method for Hg（Ⅱ） was developed. When the mixture solution of P7 and Hg（Ⅱ） was added to colloidal goldnanoparticles（GNP）, the color of GNP remained unchanged. On the contrary, the color of GNP changed from red toblue when the mixture solutions of P7 and other metal ions were added.
Bibliography:22-1183/O6
ISSN:1005-9040
2210-3171