宫颈脱落细胞人乳头瘤病毒（HPV）E6／E7mRNA检测在HPV阳性女性筛查中的应用

目的：基于临床样本探讨宫颈脱落细胞HPVE6／E7mRNA检测能否作为HPV阳性女性的筛查手段。创新点：首次比较了宫颈脱落细胞HPVE6／E7mRNA检测与宫颈脱落细胞学检测对于HPV阳性女性宫颈活检结果的预测价值。方法：所有入组的HPV阳性女性均进行宫颈脱落细胞学及HPVE6／E7mRNA检测。比较两种方法对于宫颈活检结果的预测价值。结论：对于HPV阳性的女性，高级别鳞状上皮内病变（HSIL）HPVE6／E7 mRNA阳性率高于低级别鳞状上皮内病变（LSIL）及正常病例。HPVE6／E7 mRNA与细胞学检测相比，HSIL及以上级别病变的检测敏感性（89．52％vs．86．67％，P=0．6...

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Published in浙江大学学报：B卷英文版 Vol. 18; no. 3; pp. 256 - 262
Main Author Ye-li YAO Qi-fang TIAN Bei CHENG Yi-fan CHENG Jing YE Wei-guo LU
Format Journal Article
LanguageEnglish
Published 2017
Subjects
E6／E7
HPV
mRNA
人乳头瘤病毒（HPV）
高级别鳞状上皮内病变（HSIL）
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Summary:目的：基于临床样本探讨宫颈脱落细胞HPVE6／E7mRNA检测能否作为HPV阳性女性的筛查手段。创新点：首次比较了宫颈脱落细胞HPVE6／E7mRNA检测与宫颈脱落细胞学检测对于HPV阳性女性宫颈活检结果的预测价值。方法：所有入组的HPV阳性女性均进行宫颈脱落细胞学及HPVE6／E7mRNA检测。比较两种方法对于宫颈活检结果的预测价值。结论：对于HPV阳性的女性，高级别鳞状上皮内病变（HSIL）HPVE6／E7 mRNA阳性率高于低级别鳞状上皮内病变（LSIL）及正常病例。HPVE6／E7 mRNA与细胞学检测相比，HSIL及以上级别病变的检测敏感性（89．52％vs．86．67％，P=0．671）、特异性（48．96％vs．48．96％，P=1．000）、阳性预测值（39．00％vs．38．24％，P=1．000）及阴性预测值（92．76％vs．90．97％，P=0．678）均无显著差异。HPVE6／E7mRNA检测或能成为HPV刖性女性的一种新的筛查手段。
Bibliography:Human papillomavirus （HPV）; HPV E6/E7 mRNA; High-grade squamous intraepithelial lesion （HSIL）
33-1356/Q
Cytology triage has been generally recommended for human papillomavirus （HPV）-positive women, but is highly dependent on well-trained cytologists. The present study was designed to explore whether HPV E6/E7 mRNA detection in cervical exfoliated cells can be a potential triage for HPV-positive women from a clinic-based population. Both the primary HPV testing and Papanicolaou （Pap） test were performed on all eligible HPV-positive women. HPV E6/E7 mRNA was detected by QuantiVirus HPV E6/E7 mRNA assay in cervical exfoliated cells. All HPV-positive women underwent colposcopy and further biopsy if indicated. The data were assessed by Pearson＇s Chi-squared test and the receiver operating characteristic curve. A total of 404 eligible HPV-positive women were enrolled. Positive rate of E6/E7 mRNA in high-grade squamous intraepithelial lesion （HSIL） cases was higher than that in low-grade squamous intraepithelial lesion （LSIL） or normal cases. There was no statistical difference found between mRNA and cytological testing with sensitivity （89.52% vs. 86.67%, P=0.671）, specificity （48.96% vs. 48.96%, P=1.000）, positive predictive value （39.00% vs. 38.24%, P=1.000）, and negative predictive value （92.76% vs. 90.97%, P=-0.678） for detecting ≥HSIL. HPV E6/E7 mRNA detection in cervical exfoliated cells shows the same performance as Pap triage for HSIL identification for HPV-positive women. Detection of HPV E6/E7 mRNA may be used as a new triage option for HPV-positive women.
ISSN:1673-1581
1862-1783