基于DNA条形码的物种丰富度估计:以宿迁地区鳞翅目蛾类为例

为了探究基于DNA条形码方法量化物种多样性指标的可行性,本研究以江苏省宿迁地区蛾类群落为例,基于DNA条形码方法估计群落物种丰富度并绘制等级多度分布曲线(rank-abundance curves),同时与基于传统形态学的对应指标进行比较。结果表明:(1)基于DNA条形码的物种丰富度估计与基于形态的物种丰富度估计之间没有显著差异;(2)基于形态和DNA条形码的等级多度分布曲线趋势一致,通过K-S检测发现二者之间没有显著性差异(P〉0.05)。结果显示,基于DNA条形码的物种丰富度估计能够在一定程度上补充基于形态学的方法,可以尝试将其应用于蛾类群落生态学调查研究中。...

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Bibliographic Details
Published in生物多样性 Vol. 24; no. 11; pp. 1296 - 1305
Main Author 金倩 陈芬 罗桂杰 蔡卫佳 刘旭 王昊 杨采青 郝梦迪 张爱兵
Format Journal Article
LanguageChinese
Published 2016
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Summary:为了探究基于DNA条形码方法量化物种多样性指标的可行性,本研究以江苏省宿迁地区蛾类群落为例,基于DNA条形码方法估计群落物种丰富度并绘制等级多度分布曲线(rank-abundance curves),同时与基于传统形态学的对应指标进行比较。结果表明:(1)基于DNA条形码的物种丰富度估计与基于形态的物种丰富度估计之间没有显著差异;(2)基于形态和DNA条形码的等级多度分布曲线趋势一致,通过K-S检测发现二者之间没有显著性差异(P〉0.05)。结果显示,基于DNA条形码的物种丰富度估计能够在一定程度上补充基于形态学的方法,可以尝试将其应用于蛾类群落生态学调查研究中。
Bibliography:To explore the feasibility of assessing species diversity using DNA barcoding, we investigated this approach by focusing on moths species (Lepidoptera) in Suqian, China. The study evaluated community spe- cies richness and rank-abundance curves using the DNA barcoding method, and compared it with the tradi- tional morphology method. Results indicated that there was no significant difference between the DNA barcode-based approach and the morphology-based approach. All DNA barcode-based rank-abundance curves gave similar and clear patterns when compared with morphology-based curves (Kohnogorov-Smimov two sample test, P 〉 0.05). Our results indicate that the DNA barcode-based approach is able to be used to estimate species richness.
Qian Jin1' 2, Fen Chen1, Guijie Luo1, Weijia Cail, Xu Liu1, Hao Wang1, Caiqing Yang2, Mengdi Hao2, Aibing Zhang2. (1 Suqian Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Suqian, Jiangsu 223800 2 College of Life Sciences, Capital Normal University, Beiji
ISSN:1005-0094