Transcriptome response of wheat Norin 10 to long-term elevated CO_2 under high yield field condition

• Published in: 农业科学学报：英文版 no. 9; pp. 2142 - 2152
• Main Author: LIN Yue-bing SHEN Cheng-guo LIN Er-da HAO Xing-yu HAN Xue
• Format: Journal Article
• Language: English
• Published: 2016
• Subjects: RT-PCR分析; 二氧化碳浓度; 农林; 反应; 小麦品种; 植物生长发育; 转录组; 高产田
• ISSN: 2095-3119; 2352-3425

The increasing atmospheric carbon dioxide concentration, caused by fossil fuel combustion and deforestation, plays an important role in plant growth and development. Wheat, as a major staple crop, adapts to climate change by tuning its inherent molecular mechanism, which is not well understood. The present study employed the RNA-Seq method to generate transcriptome profiles of the wheat Norin 10 in response to elevated CO_2 in comparison with ambient CO_2. The 10 895 787 high-quality clean reads of Norin 10 were assembled de novo using Trinity（without a reference genome） resulting in a total of 18 206 candidate transcripts with significant BLAST matches. GO enrichment analysis of Norin 10 at different CO_2 concentrations showed that some functional genes related to plastids, precursor metabolites, and energy, thylakoid and photosynthesis were apparently enriched at elevated CO_2（550 μmol mol^–1） in contrast to that at ambient CO_2（400 μmol mol^–1）; these findings were further confirmed by RT-PCR analysis. The findings demonstrated the specific effects of elevated CO_2 during long-term period in free air CO_2 enrichment（FACE） on transcriptome response of the high yielding wheat variety, Norin 10, which has a large spike.