Microarray-based Screening of Target Genes Regulated by Heat Shock Factor AtHsfAla in Arabidopsis thaliana

• Published in: 农业生物技术：英文版 Vol. 5; no. 3; pp. 1 - 5
• Main Author: Lihong GUO Enyin YANG Nian LI Ya XU Jieyuan ZENG
• Format: Journal Article
• Language: English
• Published: 2016
• Subjects: 信号转导通路; 因子; 基因芯片; 差异表达基因; 拟南芥; 热休克; 目标基因; 筛选
• ISSN: 2164-4993

[ Objective] This study aimed to screen target genes regulated by heat shock factor AtHsfAla in Arabidopsis thaliana. [ Method] Using AtHsfAla-in- serted mutant athsfala （SALK-068042） and wild-type A. thaliana seedlings as experimental materials, target genes regulated by heat shock factor AtHsfAla were screened by microarray assay. Differentially expressed genes were screened by multiple method. Specific functions of differentially expressed genes were analyzed by gene ontology （GO） analysis. Signal transduction pathways, in which differentia｜ly expressed genes were involved, were analyzed by pathway analysis. Gene-gene interaction network was constructed by Signal-Net. [ Result] A total of 3 672 differentially expressed genes were screened out. Up-regulated differentially expressed genes were involved in 198 functions and 7 signal transduction pathways; down-regulated differentially expressed genes were involved in 94 functions and 10 signal transduction pathways. In the signal transduction network, it was found that cwlNV4 and HXK3 had relatively high ability of mediation; AT1 G14240 and cwlNV4 ex- hibited the most interactions with other genes, which were located in key positions throughout the gene-gene interaction network. [ Conclusion] Heat shock factor AtHsfAla regulates a large number of target genes in A. thaliana.