SSc患者外周血外泌体通过microRNA-21介导成纤维细胞活化

目的比较系统性硬皮病患者（systemicsclerosis，SSc）与正常人外周血外泌体（exosome）中microRNA-21-Sp（miR-21—5p）的表达差异，评估外周血中外泌体对人皮肤成纤维细胞功能状态的影响。方法提取SSc患者及正常人外周血外泌体，荧光定量PCR法检测miR-21-Sp表达情况；外泌体刺激人皮肤成纤维细胞，CCK-8法检测细胞增殖能力，免疫印迹检测CollagenⅠ，Ⅲ及平滑肌肌动蛋白（α—SMA）水平。结果SSc患者外周血外泌体中miR-21-Sp显著高于正常人。与正常人相比，患者外泌体可明显增强人皮肤成纤维细胞的增殖能力及上调CollagenⅠ，Ⅲ，理-SM...

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Published in中国皮肤性病学杂志 Vol. 30; no. 7; pp. 661 - 664
Main Author 张淑娟 刘玉梅 麦憬霆 谭国珍
Format Journal Article
LanguageChinese
Published 2016
Subjects
外泌体
皮肤成纤维细胞
硬皮病
肌成纤维细胞
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Summary:目的比较系统性硬皮病患者（systemicsclerosis，SSc）与正常人外周血外泌体（exosome）中microRNA-21-Sp（miR-21—5p）的表达差异，评估外周血中外泌体对人皮肤成纤维细胞功能状态的影响。方法提取SSc患者及正常人外周血外泌体，荧光定量PCR法检测miR-21-Sp表达情况；外泌体刺激人皮肤成纤维细胞，CCK-8法检测细胞增殖能力，免疫印迹检测CollagenⅠ，Ⅲ及平滑肌肌动蛋白（α—SMA）水平。结果SSc患者外周血外泌体中miR-21-Sp显著高于正常人。与正常人相比，患者外泌体可明显增强人皮肤成纤维细胞的增殖能力及上调CollagenⅠ，Ⅲ，理-SMA的水平，上述作用可被转染miR-21-5p抑制剂阻断。结论SSc患者循环外泌体通过转运miR-21．5p介导人皮肤成纤维细胞向肌成纤维细胞分化。miR-21—5p可能是SSc潜在的生物标记物。
Bibliography:Systemic sclerosis ; Exosome ; Skin fibroblast ; Myofibroblast
Objective To explore the levels of microRNA-21-5p （miR-21-5p） in circulating exosome respectively isolated from systemic sclerosis （SSc） patients and healthy volunteers. The effects of exosome on skin fibroblasts were also evaluated. Methods Specific kits were used for circulating exosome isolation and RNA extraction. Levels of miR-21-5p were detected by qRT-PCR. CCK-8 was used to evaluate proliferation ability. Western blots were used to evaluate the protein levels of collagen I , collagen Ⅲ and a-smooth muscle actin （α-SMA）. Results The levels of miR-21-5p were much higher in circulating exosome from SSc patients than that in healthy volunteers. Compared to healthy control, exosome from SSe patients could significantly elevated protein levels of collagen I , Ⅲ and α-SMA in skin fibroblasts, which could be partly blocked by miR-21-Sp transfection. Conclusion Circulating exosome isolated from SSc patients could promote fibroblasts transforming into
ISSN:1001-7089