Synergetic effects of ciliary neurotrophic factor and olfactory ensheathing cells on optic nerve reparation（complete translation）

• Published in: 中国神经再生研究：英文版 Vol. 11; no. 6; pp. 1006 - 1012
• Main Author: Dan-ping Yin Qing-ying Chen Lin Liu
• Format: Journal Article
• Language: English
• Published: 2016
• Subjects: CNTF; 协同作用; 嗅鞘细胞; 因子和; 治疗; 睫状神经营养因子; 视神经损伤; 视网膜神经节细胞
• ISSN: 1673-5374

At present, there is no effective treatment for the repair of the optic nerve after injury, or improvement of its microenvironment for regeneration. Intravitreally injected ciliary neurotrophic factor（CNTF） and olfactory ensheathing cells（OECs） promote the long-distance regrowth of severed optic nerve fibers after intracranial injury. Here, we examined the efficacy of these techniques alone and in combination, in a rat model of optic nerve injury. We injected condensed OEC suspension at the site of injury, or CNTF into the vitreous body, or both simultaneously. Retrograde tracing techniques showed that 4 weeks postoperatively, the number of surviving retinal ganglion cells and their axonal density in the optic nerve were greater in rats subjected to OEC injection only than in those receiving CNTF injection only. Furthermore, combined OEC ＋ CNTF injection achieved better results than either monotherapy. These findings confirm that OECs are better than CNTF at protecting injured neurons in the eye, but that combined OEC and CNTF therapy is notably more effective than either treatment alone.