3种抑制剂对褐色橘蚜乙酰胆碱酯酶活性的影响
乙酰胆碱酯酶（Acetylcholinesterase,ACh E;EC3.1.1.7）作为有机磷和氨基甲酸酯类杀虫剂的作用靶标,一直是昆虫毒理学领域研究的热点。本研究采用微量滴度酶标板法研究了褐色橘蚜Toxoptera citricida不同发育阶段ACh E的生化特性及个体活性频率,离体条件下分析了3种药剂对ACh E的影响。结果表明,无翅成蚜对底物碘化硫代乙酰胆碱亲和力显著高于若蚜;就个体活性分布频率而言,25%-30%的无翅成蚜分布在9.5-10.5 nmol/（min·insect）区间,而50%-55%的若蚜分布在2-3 nmol/（min·insect）区间;离体条件下,毒扁豆碱...
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| Published in | 环境昆虫学报 Vol. 38; no. 2; pp. 401 - 407 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
2016
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1674-0858 |
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| Summary: | 乙酰胆碱酯酶（Acetylcholinesterase,ACh E;EC3.1.1.7）作为有机磷和氨基甲酸酯类杀虫剂的作用靶标,一直是昆虫毒理学领域研究的热点。本研究采用微量滴度酶标板法研究了褐色橘蚜Toxoptera citricida不同发育阶段ACh E的生化特性及个体活性频率,离体条件下分析了3种药剂对ACh E的影响。结果表明,无翅成蚜对底物碘化硫代乙酰胆碱亲和力显著高于若蚜;就个体活性分布频率而言,25%-30%的无翅成蚜分布在9.5-10.5 nmol/（min·insect）区间,而50%-55%的若蚜分布在2-3 nmol/（min·insect）区间;离体条件下,毒扁豆碱、残杀威、异丙威等3种杀虫剂对褐色橘蚜ACh E有显著的抑制作用,与成蚜相比,若蚜ACh E对3种药剂更敏感,而毒扁豆碱对褐色橘蚜无翅成蚜与若蚜ACh E的抑制作用最好。本研究表明褐色橘蚜成蚜与若蚜生化特性及对药剂的敏感性存在显著差异。 |
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| Bibliography: | MOU Xing, WANG Jin-Jun （College of Plant Protection, Southwest University, Key Laboratory of Entomology and Pest Control Engineering, Chongqing 400716, China） Toxoptera citricida; ACh E; kinetics; in vitro inhibition 44-1640/Q Acetylcholinesterase（ ACh E） plays an important role in the nervous impulse of natural species.The biochemical and toxicological characteristics of ACh E from Toxoptera citricida wingless adult and nymph were studied in the laboratory applying microplate reader assay. The results showed that ACh E from wingless adult aphid on substrate affinity was significantly higher than that of nymphs. As for the individual distribution of ACh E activity in frequency,25%- 30% of wingless adults are distributed in the range of9. 5- 10. 5 nmol /（ min · insect）,50%- 55% of nymphs are focused on the range of 2- 3 nmol /（ min·insect）. Besides,all three kinds of inhibitors significantly inhibited ACh E from T. citricida wingless adults and nymphs. Compared to the counterparts of wingless adults,ACh E from ny |
| ISSN: | 1674-0858 |