转基因大豆MON89788基体标准物质的研制

转基因产品成分检测是转基因生物安全管理和标识的重要支撑,而转基因生物标准物质是获得准确、可靠、具有可比性检测结果的保证。转基因大豆（Glycine max）MON89788为我国批准进口用作加工原料的农业转基因生物,因此,制备转基因大豆MON89788基体标准物质是对其进行安全管理所必需的。本研究通过将转基因大豆MON89788和其受体材料A3244分别冷冻研磨成粉末,按质量分数称量配制,得到转基因含量为49.3 g/kg的标准物质。采用方差分析法（F检验法）进行均匀性检验,统计量F值为1.618,小于临界值F_（（0.05,11,24））,标准物质均匀性良好;短期稳定性考察结果表明,标准物质...

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Bibliographic Details
Published in农业生物技术学报 Vol. 24; no. 2; pp. 295 - 304
Main Author 张丽 宋贵文 沈平 李文龙 武玉花 吴刚
Format Journal Article
LanguageChinese
Published 2016
Subjects
MON89788
qRT-PCR
基体标准物质
定值
转基因大豆
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Summary:转基因产品成分检测是转基因生物安全管理和标识的重要支撑,而转基因生物标准物质是获得准确、可靠、具有可比性检测结果的保证。转基因大豆（Glycine max）MON89788为我国批准进口用作加工原料的农业转基因生物,因此,制备转基因大豆MON89788基体标准物质是对其进行安全管理所必需的。本研究通过将转基因大豆MON89788和其受体材料A3244分别冷冻研磨成粉末,按质量分数称量配制,得到转基因含量为49.3 g/kg的标准物质。采用方差分析法（F检验法）进行均匀性检验,统计量F值为1.618,小于临界值F_（（0.05,11,24））,标准物质均匀性良好;短期稳定性考察结果表明,标准物质可在常温下稳定储存4周,在-20℃下长期稳定性达到30个月。本批MON89788基体标准物质有两个特性量值,一是基于重量法配比混合的粉末质量分数,量值为49.3 g/kg,扩展不确定度为2.0 g/kg;二是基于q RT-PCR 8家实验室联合定值的转基因DNA与总DNA的质量分数,量值为49.8 ng/μg,扩展不确定度为5.9 ng/μg。本批标准物质每瓶1.0 g,使用时最小取样量为100 mg,可用于对转基因大豆MON89788进行定性和定量检测,以及转基因大豆MON89788特异性检测方法的评价和实验室质量控制。本研究为我国转基因检测基体标准物质的研制提供了理论基础和技术支持。
Bibliography:Genetically modified soybean; MON89788; Matrix reference material; Value characterization; qRT-PCR
Genetically modified organism（GMO） ingredients detection is an important part of GMO safety management and GMO labeling,GMO detection reference material（RM） is guaranteed to get accurate,reliable,comparable GMO ingredients detection results. GM soybean（Glycine max） MON89788 is a GM plant that has been approved to import and use as in-process raw material in China. Therefore,preparation of GM soybean MON89788 RM is essential for its safety management. In this study,GM soybean MON89788 and traditional soybean seeds A3244 were milled using a cryo-grinding vibrating mill separately,then the GM soybean MON89788 matrix RM was prepared by weighting and mixing of GM and Non- GM powder. The value of MON89788 matrix RM was 49.3 g/kg in mass fraction. The mixture was further dispensed into brown bottles. Analysis of variance（F test） was performed to test homogeneity of RMs. The calculated Fstatistics is 1.618,less than the
ISSN:1674-7968