外源激素处理后家蚕吡哆醛激酶和磷酸吡哆醇氧化酶转录水平的变化

【目的】研究家蚕Bombyx mori经蜕皮激素（20-hydroxyecdysone,20-E）和保幼激素类似物（juvenile hormone analogue,JHA）处理后引起吡哆醛激酶（pyridoxal kinase,PLK）和磷酸吡哆醇氧化酶（pyridoxine-5＇-phosphate oxidase,PNPO）的转录水平变化,为进一步研究激素对蚕体营养代谢等工作奠定基础。【方法】以20-E和JHA分别喂食不同发育时期（5龄第1,3和5天）的家蚕幼虫,以喂食蒸馏水的家蚕为对照,采用实时荧光定量PCR（realtime quantitative PCR）方法在处理后24和48...

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Bibliographic Details
Published in昆虫学报 Vol. 58; no. 12; pp. 1285 - 1290
Main Author 杨欢欢 姚丽丽 张剑韵 黄龙全
Format Journal Article
LanguageChinese
Published 2015
Subjects
保幼激素类似物
吡哆醛激酶
家蚕
磷酸吡哆醇氧化酶
蜕皮激素
转录水平
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Summary:【目的】研究家蚕Bombyx mori经蜕皮激素（20-hydroxyecdysone,20-E）和保幼激素类似物（juvenile hormone analogue,JHA）处理后引起吡哆醛激酶（pyridoxal kinase,PLK）和磷酸吡哆醇氧化酶（pyridoxine-5＇-phosphate oxidase,PNPO）的转录水平变化,为进一步研究激素对蚕体营养代谢等工作奠定基础。【方法】以20-E和JHA分别喂食不同发育时期（5龄第1,3和5天）的家蚕幼虫,以喂食蒸馏水的家蚕为对照,采用实时荧光定量PCR（realtime quantitative PCR）方法在处理后24和48 h对各组幼虫后部丝腺中PLP合成酶PLK和PNPO的转录水平进行分析。【结果】5龄第1天幼虫经20-E处理24和48 h后,PLK和PNPO的转录水平出现上调且与对照的差异达到极显著（P〈0.01）;5龄第3天幼虫经20-E处理,PLK的转录水平在48 h出现下调且与对照的差异达到显著（P〈0.05）,PNPO的转录水平在24和48 h均出现上调且与对照的差异达到极显著（P〈0.01）;5龄第5天幼虫经20-E处理后PLK和PNPO的转录水平无变化。5龄第1天幼虫经JHA处理后PLK和PNPO的转录水平未受到影响;5龄第3天幼虫经JHA处理后,PLK的转录水平在48 h出现显著下调且与对照的差异达到显著（P〈0.05）,PNPO的转录水平在24和48 h后均出现显著下调且与对照的差异达到极显著（P〈0.05）;5龄第5天幼虫经JHA处理24和48 h后,PLK和PNPO的转录水平出现下调且与对照的差异达到极显著（P〈0.01）。【结论】20-E和JHA显著影响家蚕5龄幼虫PLK和PNPO的转录水平,20-E提高5龄前期家蚕PLK和PNPO的转录水平,JHA降低5龄后期它们的转录水平,为深入研究激素对VB_6的调控奠定基础。
Bibliography:11-1832/Q
[ Aim ] To further research the hormone regulation of nutrition metabolism, we analyzed the transcription levels of pyridoxal kinase （PLK） and pyridoxine-5 ＇-phosphate oxidase （PNPO） of the silkworm, Bornbyx mori, treated with 20-hydroxyecdysone （20-E） and juvenile hormone analogue （JHA）. [Methods] The day-l, day-3 and day-5 5th instar larvae of B. mori were fed with 20-E or JHA, and those fed on distilled water were used as the control. We assayed the transcriptional levels of PLK and PNPO in silk glands of the treated larvae at 24 and 48 h post treatment by using real-time quantitative PCR. [Results] In the day-1 5th instar larvae treated with 20-E for 24 and 48 h, respectively, the transcriptional levels of PLK and PNPO significantly increased compared with the control （P 〈 0.01 ）. In the day-3 5th instar larvae treated by 20-E, the transcriptional level of PLK decreased compared with the control at 48 h after treatment （ P 〈 0.05 ）, whereas the transcriptional level of PNPO significantly increase
ISSN:0454-6296