姜黄素对成纤维细胞中TGF-β1、Smad3及Smad7表达的影响

目的探讨姜黄素对人皮肤成纤维细胞（HSF）生长的抑制作用及可能机制。方法体外培养HSF,给予不同浓度的姜黄素作用不同的时间;采用MTT法检测HSF存活率,采用流式细胞术检测细胞周期的变化情况,采用RT-PCR和Western blotting分别检测细胞内转化生长因子-β1（TGF-β1）、Smad3和Smad7的m RNA和蛋白水平的表达。结果 40μmol/L姜黄素处理,使HSF的存活率显著降低（P〈0.001）;处于S期的细胞数减少,G1期的细胞数量增加（P〈0.001）;TGF-β1及其下游因子Smad3的m RNA和蛋白水平的表达也明显受到抑制,而Smad7 m RNA和蛋白水平的表...

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Published in上海交通大学学报：医学版 Vol. 35; no. 12; pp. 1814 - 1818
Main Author 秦开秀 简华刚
Format Journal Article
LanguageChinese
Published 2015
Subjects
Smad3
Smad7
姜黄素
皮肤成纤维细胞
转化生长因子β1
Online AccessGet full text
ISSN1674-8115

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Summary:目的探讨姜黄素对人皮肤成纤维细胞（HSF）生长的抑制作用及可能机制。方法体外培养HSF,给予不同浓度的姜黄素作用不同的时间;采用MTT法检测HSF存活率,采用流式细胞术检测细胞周期的变化情况,采用RT-PCR和Western blotting分别检测细胞内转化生长因子-β1（TGF-β1）、Smad3和Smad7的m RNA和蛋白水平的表达。结果 40μmol/L姜黄素处理,使HSF的存活率显著降低（P〈0.001）;处于S期的细胞数减少,G1期的细胞数量增加（P〈0.001）;TGF-β1及其下游因子Smad3的m RNA和蛋白水平的表达也明显受到抑制,而Smad7 m RNA和蛋白水平的表达却明显增加,并呈现浓度依赖性（P〈0.001）。结论姜黄素通过上调Smad7、下调Smad3的m RNA和蛋白表达而抑制TGF-β1信号通路,进而抑制HSF增殖作用,将为临床改善创面愈合提供新的思路。
Bibliography:QIN Kai-xiu;JIAN Hua-gang（ Department of Emergency, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China）
Objective To explore the inhibitory effect of curcumin on the growth of human skin fibroblast（HSF） and possible mechanisms.Methods HSF were cultured in vitro and treated with different concentrations of curcumin for different time periods.The viability of HSF was detected by MTT,changes of cell cycle were detected by flow cytometry;and mRNA and protein expressions of transforming growth factor-β（TGF-β1）,Smad3,and Smad7 were detected by RT-PCR and Western blotting.Results The viability of HSF treated with curcumin of 40 μmol/L obviously decreased（P〈0.001）.The curcumin of 40 μmol/L significantly decreased the number of cells in the S phase but increased the number of cells in the G_1 phase（P〈0.001）.Furthermore,mRNA and protein expressions of TGF-β1 and its downstream factor Smad3 were significantly inhibited,while mRNA and protein expressions of Smad7 significantly increased
ISSN:1674-8115