利用表达谱芯片分析水稻籼粳交杂种优势的分子基础

[目的]本文旨在研究水稻籼粳交杂种优势的分子基础.[方法]利用Affymetrix水稻表达谱芯片分析了强优势水稻籼粳交组合‘申9A/繁31’与母本‘申9A’和父本‘繁31’在幼穗分化初期剑叶组织的基因表达差异,并对差异表达基因进行了基于生物学过程的GO（gene ontology）功能分类和代谢通路分析.[结果]幼穗分化初期杂种与双亲的表型已经产生了明显差异;共有2 258个基因在杂种与亲本之间差异表达（差异倍数≥2或≤0.5）,杂种与双亲均显示差异表达的基因有327个,杂种与父本之间的差异表达基因远多于母本.杂种差异表达基因具有多种表达模式,具有正效应基因的增强表达和具有负效应基因的抑制表达...

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Bibliographic Details
Published in南京农业大学学报 Vol. 38; no. 6; pp. 883 - 889
Main Author 牛付安 曹黎明 储黄伟 程灿 周继华 罗小金 顾永平 袁勤 罗忠永
Format Journal Article
LanguageChinese
Published 2015
Subjects
分子基础
杂种优势
水稻
表达谱芯片
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ISSN1000-2030

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Summary:[目的]本文旨在研究水稻籼粳交杂种优势的分子基础.[方法]利用Affymetrix水稻表达谱芯片分析了强优势水稻籼粳交组合‘申9A/繁31’与母本‘申9A’和父本‘繁31’在幼穗分化初期剑叶组织的基因表达差异,并对差异表达基因进行了基于生物学过程的GO（gene ontology）功能分类和代谢通路分析.[结果]幼穗分化初期杂种与双亲的表型已经产生了明显差异;共有2 258个基因在杂种与亲本之间差异表达（差异倍数≥2或≤0.5）,杂种与双亲均显示差异表达的基因有327个,杂种与父本之间的差异表达基因远多于母本.杂种差异表达基因具有多种表达模式,具有正效应基因的增强表达和具有负效应基因的抑制表达对杂种优势均有贡献.差异表达基因主要参与了磷代谢、运输、防卫反应以及碳水化合物代谢等生物学过程,并在碳固定、氨基酸代谢、糖酵解/糖原异生以及磷酸肌醇代谢等多个代谢通路富集.[结论]水稻籼粳交杂种优势存在复杂的分子基础,多种分子机制参与了杂种优势的形成,光合效率和光合产物运输分配效率的提高可能是杂种优势产生的主要原因.
Bibliography:[Objectives]The aim of the paper is to study the molecular basis of heterosis in indica and japonica hybrid rice. [Methods]Gene transcriptional profiles of flag leaves at early panicle differentiation stage of an indica and japonica hybrid rice‘Shen9A/Fan31’and its parents‘Shen9A’and‘Fan31’were analyzed by using Affymetrix rice expression microarray. GO（gene ontology）functional category based on biological process and metabolic pathway analysis were further performed for differentially expressed genes（DGs）. [Results]The results showed that the phenotypes of the hybrid were significantly different from its parents at early panicle differentiation stage. A total of 2 258 DGs between the hybrid and its parents were identified（fold change≥2 or ≤0.5）,of which 327 genes were differentially expressed between both the hybrid and the maternal and paternal parents. Numbers of DGs between the hybrid and paternal parent were far more than those between the hybrid and maternal parent. The DGs of hybrid were expressed in v
ISSN:1000-2030