HPLC法在测定番泻总苷胶囊中番泻苷AB含量中的应用

目的建立番泻总苷胶囊中番泻苷A、B含量的测定方法。方法采用高效液相色谱（HPLC）法,以八烷基硅烷键合硅胶柱、乙腈-0.1%三氟乙酸水溶液为流动相梯度洗脱,检测波长340 nm。结果番泻苷A在0.210 4~1.052 0μg、番泻苷B在0.225 0~1.125 0μg呈良好的线性关系,平均回收率分别为99.51%（RSD=1.30%）和99.07%（RSD=0.69%）。结论该方法简单快捷、结果可靠,可作为番泻总苷胶囊质量控制的方法。...

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Bibliographic Details
Published in现代医药卫生 Vol. 31; no. 21; pp. 3235 - 3237
Main Author 张苗 顾燕
Format Journal Article
LanguageChinese
Published 2015
Subjects
番泻叶（中药）
番泻甙/分析
胶囊
色谱法,高压液相
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Summary:目的建立番泻总苷胶囊中番泻苷A、B含量的测定方法。方法采用高效液相色谱（HPLC）法,以八烷基硅烷键合硅胶柱、乙腈-0.1%三氟乙酸水溶液为流动相梯度洗脱,检测波长340 nm。结果番泻苷A在0.210 4~1.052 0μg、番泻苷B在0.225 0~1.125 0μg呈良好的线性关系,平均回收率分别为99.51%（RSD=1.30%）和99.07%（RSD=0.69%）。结论该方法简单快捷、结果可靠,可作为番泻总苷胶囊质量控制的方法。
Bibliography:Zhang Miao,Gu Yan （1. Jiangs u Provincial Hospital of Traditional Chinese Medicine, Nanjing , Jiangsu 210029, China; 2. Jiansu Ruibo Pharmaceutical Co. ,Ltd. ,Nanjing ,Jiangsu 211112, China）
Cassiae angustifolia（TCD）; Capsules; Sennoside/analysis; Chromatography; high pressure liquid
Objective To establish a method for the determination of sennoside A and B contents in Senna Total Glyco-sides Capsule. Methods The high performance liquid chromatography（HPLC） method was adopted,the gradient elution was performed with octadecyl silane bonded silica gel column,the mobile phase of acetonitrile-0.1% trifluoroacetic acid aqueous solution and the detection wavelength of 340 nm. Results Sennoside A in the range of 0.210 4-1.052 0 μg and sennoside B in the range of 0.225 0-1.125 0 μg had good linear relationship. The average recovery rates were 99.51%（RSD=1.30%） for sennoside A and 99.07%（RSD =0.69%） for sennoside B. Conclusion The established method is simple,quick and reliable,which can be used as a quality control met
ISSN:1009-5519