优质晚抽薹四倍体不结球白菜的创制及特性

[目的]用秋水仙素诱导晚抽薹不结球白菜二倍体,以期创制抽薹晚、优质、高产的不结球白菜四倍体新种质。[方法]采用1.5和2.0 g·L-1秋水仙素分别对二倍体不结球白菜茎尖生长点进行4次和6次点滴处理,并鉴定筛选同源四倍体。利用实时荧光定量（qRT-PCR）技术检测抽薹基因的表达,测定营养品质和农艺性状,筛选晚抽薹且优质四倍体材料。[结果]用2.0 g·L-1秋水仙素处理二倍体茎尖6次的效果最好,四倍体的诱导率为5.90%;与二倍体相比,四倍体的叶片、花器、角果和种子都呈现明显差异。流式细胞仪鉴定二倍体（2n=2x=20）DNA相对含量为200,四倍体（2n=4x=40）为400。四倍体抽薹时间...

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Bibliographic Details
Published in南京农业大学学报 Vol. 38; no. 5; pp. 757 - 763
Main Author 户秋稳 吕炜 张蜀宁 李艳艳 侯喜林 刘惠吉
Format Journal Article
LanguageChinese
Published 2015
Subjects
RT-PCR
不结球白菜
农艺性状
四倍体
晚抽薹
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Summary:[目的]用秋水仙素诱导晚抽薹不结球白菜二倍体,以期创制抽薹晚、优质、高产的不结球白菜四倍体新种质。[方法]采用1.5和2.0 g·L-1秋水仙素分别对二倍体不结球白菜茎尖生长点进行4次和6次点滴处理,并鉴定筛选同源四倍体。利用实时荧光定量（qRT-PCR）技术检测抽薹基因的表达,测定营养品质和农艺性状,筛选晚抽薹且优质四倍体材料。[结果]用2.0 g·L-1秋水仙素处理二倍体茎尖6次的效果最好,四倍体的诱导率为5.90%;与二倍体相比,四倍体的叶片、花器、角果和种子都呈现明显差异。流式细胞仪鉴定二倍体（2n=2x=20）DNA相对含量为200,四倍体（2n=4x=40）为400。四倍体抽薹时间比二倍体晚。RT-PCR结果表明：在二、四倍体中Bc FLC3和FRI基因表达随春化时间的延长下调,而VIN3表达上调,四倍体始终高于二倍体;四倍体可溶性糖、可溶性蛋白、维生素C、游离氨基酸和硒含量分别比二倍体显著增加了75.63%、23.84%、44.66%、47.47%和53.79%,有机酸、纤维素和干物质百分比分别降低10.67%、17.56%和12.5%;四倍体单株质量、开展度、叶宽、叶质量、十叶厚、叶柄长和短缩茎质量7个农艺性状与二倍体差异显著,产量增加11.13%。[结论]四倍体不结球白菜表现出营养价值高、农艺性状好且晚抽薹的特性。
Bibliography:non-heading Chinese cabbage; tetraploid; late-bolting; RT-PCR; agronomic traits
HU Qiuwen , LU Wei ,ZHANG Shuning, LI Yanyan, HOU Xilin, LIU Huiji （ 1. College of Horticulture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;2. Nanjing Ideal Seedling Limited Company, Nanjing 210042, China）
[Objectives]Diploid late-bolting plants of non-heading Chinese cabbage were induced by colchicine to creat new germplasm having late-bolting character,high quality and high yield. [Methods]The stem tip growing point of diploid non-heading Chinese cabbage were dripped with 1. 5 and 2. 0 g·L-1colchicine for 4 and 6 times,respectively,then identificated and selected. Different expression levels of bolting genes were checked using RT-PCR. In addition,nutrition was measured and agronomic characters were investigated to create high quality and late-bolting tetraploid. [Results]The result showed that the most effective treatment was2.0 g·L-1for 6 times with induc
ISSN:1000-2030