激活转录因子5启动子区甲基化水平的研究

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Bibliographic Details
Published in浙江大学学报：B卷英文版 Vol. 16; no. 9; pp. 757 - 762
Main Author Xiao-min HUA Juan WANG Dong-meng QIAN Jing-yi SONG Hao CHEN Xiu-li ZHU Rui ZHOU Yu-dan ZHAO Xiu-zhi ZHOU Ling LI Li ZHANG Xu-xia SONG Bin WANG
Format Journal Article
LanguageEnglish
Published 2015
Subjects
DNA甲基化
启动子
激活转录因予
胶质瘤
表观遗传学
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Bibliography:DNA methylation, Activating transcription factor, Promoter region, Epigenetic, Glioma
33-1356/Q
Transcription factors, which represent an important class of proteins that play key roles in controlling cellular proliferation and cell cycle modulation, are attractive targets for cancer therapy. Previous researches have shown that the expression level of activating transcription factor 5 （ATF5） was frequently increased in glioma and its acetylation level was related to glioma. The purposes of this study were to explore the methylation level of ATF5 in clinical glioma tissues and to explore the effect of ATF5 methylation on the expression of ATF5 in glioma. Methylation of the promoter region of ATF5 was assayed by bisulflte-specific polymerase chain reaction （PCR） sequencing analysis in 35 cases of glioma and 5 normal tissues. Quantitative real-time PCR （qRT-PCR） was also performed to detect ATF5 mRNA expression in 35 cases of glioma and 5 normal tissues. Clinical data were collected from the patients and analyzed. The percentages of methylation of the ATF5 gene in the promoter region in healthy control, patients with well-differentiated glioma, and those with poorly differentiated glioma were 87.78%, 73.89%, and 47.70%, respectively. Analysis of the methylation status of the promoter region of the ATF5 gene showed a gradually de- creased methylation level in poorly differentiated glioma, well-differentiated glioma, and normal tissues （P〈0.05）. There was also a significant difference between well-differentiated glioma and poorly differentiated glioma （P〈0.05）. ATF5 mRNA expression in glioma was significantly higher than that in the normal tissues （P〈0.05）. This study provides the first evidence that the methylation level of ATF5 decreased, and its mRNA expression was evidently up-regulated in glioma.
ISSN:1673-1581
1862-1783