Effect of Bushenwenyanghuayu decoction on nerve growth factor and bradykinin/bradykinin B_1 receptor in a endometriosis dysmenorrhea mouse model

• Published in: 中医杂志：英文版 no. 2; pp. 184 - 191
• Main Author: Chen Jingwei Du Huilan Tong Ruixiao Yang Hua Ma Huirong
• Format: Journal Article
• Language: English
• Published: 2015
• Subjects: experimental; expression; real-time; response; 因子和; 子宫内膜异位症; 神经生长; 缓激肽
• ISSN: 0255-2922

OBJECTIVE：To observe the effects of Bushenwenyanghuayu decoction（BD）,a Traditional Chinese Medicine（TCM）,on the serum concentration of nerve growth factor（NGF） and bradykinin（BK）,and protein and mRNA levels of NGF and bradykinin B_1receptor（BKB1R） in a mouse model of endometriosis dysmenorrhea.METHODS：Seventy-five experimental female BALB/c mice were randomly divided into five groups,15 mice each：sham,model,BD high dose（61.67 g/kg）,BD low dose（15.42 g/kg）,and gestrinone（0.4 mg/kg） groups.All the mice except for those in the sham group underwent auto-transplantation surgery and were gavaged estradiol valerate（0.5 mg/kg,daily for 12 days） after surgery.On the 12 th day,1 h after administration,writhing response was induced by intraperitoneal injection of oxytocin at 2 U/mouse.The writhing frequency and latency were recorded and the volume of the ectopic foci was measured.The concentration of serum NGF and BK was detected by enzyme-linked immunosorbent assay,the protein expression of NGF and BKB1 R was tested by immunohistochemistry and western blotting,and NGF and BKB1 R mRNAs were detected by real-time PCR.RESULTS：Compared with the model group,the volume of the ectopic foci in the treatment groups was significantly lower（P 〈 0.01）,the writhing frequency was decreased（P 〈 0.05）,and the writhing latency was prolonged（P 〈 0.01）.Compared with the sham group,serum NGF and BK levels in the model group were significantly increased（P 〈0.01）.There were positive correlations for writhing frequency among the NGF and BK groups（P 〈0.01）.The serum NGF and BK levels were significantly lower in the treatment groups than the model group（P 〈 0.05）.The protein expression of NGF,BKB1 R was significantly decreased in the treatment groups compared with the model group（P 〈 0.01）.NGF and BKB1 R mRNA expression was significantly decreased in the treatment groups compared with the model group（P 〈 0.01）.CONCLUSION：NGF and BK/BKB1 R may play an important role in the development of endometriosis-associated dysmenorrhea,and BD was found to inhibit the development of endometriosis and relieve dysmenorrhea by influencing NGF and BK/BKB1 R mRNA and protein levels.