基于线粒体基因组D-loop区全序列分析安义瓦灰鸡遗传多样性及其起源进化关系

本研究旨在探究江西安义瓦灰鸡（Gallus gallus domestiaus）的遗传多样性和起源。对30只安义瓦灰鸡线粒体基因组（mitochondrial genome,mt DNA）D-loop区全序列的进行PCR扩增和测序（Gen Bank登录号：KP893677-KP893682）,并结合Gen Bank中红色原鸡的D-loop区序列,分析安义瓦灰鸡线粒体多态性及其起源。结果发现,安义瓦灰鸡mt DNA D-loop区全长1 231-1 232 bp,核苷酸多样性（Pi）为（0.006 13±0.000 14）,单倍型多样性（Hd）为（0.837±0.027）。在30只个体中,共发现...

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Bibliographic Details
Published in农业生物技术学报 Vol. 23; no. 7; pp. 940 - 944
Main Author 高玉时 贾晓旭 唐修君 唐梦君 樊艳凤 陆俊贤 顾荣 葛庆联 苏一军
Format Journal Article
LanguageChinese
Published 2015
Subjects
D-loop区
安义瓦灰鸡
系统发育关系
线粒体DNA
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Summary:本研究旨在探究江西安义瓦灰鸡（Gallus gallus domestiaus）的遗传多样性和起源。对30只安义瓦灰鸡线粒体基因组（mitochondrial genome,mt DNA）D-loop区全序列的进行PCR扩增和测序（Gen Bank登录号：KP893677-KP893682）,并结合Gen Bank中红色原鸡的D-loop区序列,分析安义瓦灰鸡线粒体多态性及其起源。结果发现,安义瓦灰鸡mt DNA D-loop区全长1 231-1 232 bp,核苷酸多样性（Pi）为（0.006 13±0.000 14）,单倍型多样性（Hd）为（0.837±0.027）。在30只个体中,共发现18处单核苷酸多态位点,6种单倍型。系统发育分析发现,6种单倍型可以分为A、B、C 3个分支,其中C为主要分支,有3种单倍型,A分支有1种倍型,B分支有2种倍型。A和B分支与红色原鸡滇南亚种（Gallus gallus spadiceus）聚为一类,C分支与4个原鸡亚种聚为一类。研究结果表明,安义瓦灰鸡mt DNA D-loop区有丰富的多态性,并且有多个母系起源,从分子水平上为安义瓦灰鸡的遗传资源保护和开发利用提供了参考。
Bibliography:Anyi Tile-like Chickens; Mitochondrial DNA; D-loop region; Phylogenetic relationship; Origin
11-3342/S
This study sought to assess mitochondrial DNA（mt DNA） diversity and origin of Jiangxi Anyi Tilelike Chickens（Gallus gallus domestiaus）. Blood samples were collected from 30 chickens. The complete sequence of the mt DNA D- loop was PCR amplified and subsequently sequenced（Genbank accession No.KP893677-KP893682.）. DNA sequence of mt DNA D-loop of five red jungle fowl subspecies were collected from the Gen Bank and used as reference sequences. The results showed that, the D-loop region of Anyi Tilelike Chickens was 1 231-1 232 bp in length. The nucleotide diversity was（0.006 13 ± 0.000 14）, and the haplotype diversity was（0.837±0.027）. The 6 haplotypes were observed based on 18 mutant site and clustered into three clades（A, B, and C）, of which clade C was dominant. Three haplotypes belong to clade C, two haplotypes are in clade B, one is in clade A. The clade A and clade B were clustered with Gallus gallus spadic
ISSN:1674-7968