The functional analysis of OsTDF1 reveals a conserved genetic pathway for tapetal development between rice and Arabidopsis

During anther development, the tapetum pro- vides materials and nutrients for pollen development. In Arabidopsis, several transcription factors have been iden- tified to form a genetic pathway （DYT1-TDF1-AMS- MS188-MS1） for tapetal development and function. DEFECTIVE in TAPETAL DEVELOPMENT and FUNCT...

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Published in中国科学通报：英文版 no. 12; pp. 1073 - 1082
Main Author Ci-Feng Cai Jun Zhu Yue Lou Zong-Li Guo Shuang-Xi Xiong Ke Wang Zhong-Nan Yang
Format Journal Article
LanguageEnglish
Published 2015
Subjects
保守
发育过程
拟南芥
水稻
细胞程序性死亡
绒毡层细胞
花药绒毡层
遗传
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Summary:During anther development, the tapetum pro- vides materials and nutrients for pollen development. In Arabidopsis, several transcription factors have been iden- tified to form a genetic pathway （DYT1-TDF1-AMS- MS188-MS1） for tapetal development and function. DEFECTIVE in TAPETAL DEVELOPMENT and FUNCTION1 （TDF1） is a member of the R2R3 MYB family and is essential for early tapetum development in Arabidopsis. Here, we characterized an ortholog of Ara- bidopsis TDF1 in rice, OsTDF1 （LOC_Os03g18480）. OsTDF1 shares 69 % amino acid sequence identity with AtTDF1 in the putative MYB domain near the N-terminal region. RT-PCR and in situ hybridization show that OsTDF1 is specifically expressed in tapetal cells of rice anthers. The expression of OsTDF1 in Arabidopsis tdfl mutant restores its fertility, suggesting that this homolog can fulfill the normal function of TDF1 in Arabidopsis. The ostdfl knockout mutant exhibits a male-sterile phenotype. Its tapetal cells exhibit a vacuolated and hypertrophic phenotype similar to that of Arabidopsis tdfl mutants. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assays revealed OsTDF1 acts as an essential regulator for tapetum programmed cell death. The qRT-PCR analysis revealed that OsTDF1 acts downstream of UDT1 and upstream of TDR, EAT1, OsMYBI03 and PTCI in rice, suggesting that the genetic pathway for tapetum development is generally conserved between rice and Arabidopsis,
Bibliography:Oryza sativa; Transcription factor;Tapetum ; OsTDF1
During anther development, the tapetum pro- vides materials and nutrients for pollen development. In Arabidopsis, several transcription factors have been iden- tified to form a genetic pathway （DYT1-TDF1-AMS- MS188-MS1） for tapetal development and function. DEFECTIVE in TAPETAL DEVELOPMENT and FUNCTION1 （TDF1） is a member of the R2R3 MYB family and is essential for early tapetum development in Arabidopsis. Here, we characterized an ortholog of Ara- bidopsis TDF1 in rice, OsTDF1 （LOC_Os03g18480）. OsTDF1 shares 69 % amino acid sequence identity with AtTDF1 in the putative MYB domain near the N-terminal region. RT-PCR and in situ hybridization show that OsTDF1 is specifically expressed in tapetal cells of rice anthers. The expression of OsTDF1 in Arabidopsis tdfl mutant restores its fertility, suggesting that this homolog can fulfill the normal function of TDF1 in Arabidopsis. The ostdfl knockout mutant exhibits a male-sterile phenotype. Its tapetal cells exhibit a vacuolated and hypertrophic phenotype similar to that of Arabidopsis tdfl mutants. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assays revealed OsTDF1 acts as an essential regulator for tapetum programmed cell death. The qRT-PCR analysis revealed that OsTDF1 acts downstream of UDT1 and upstream of TDR, EAT1, OsMYBI03 and PTCI in rice, suggesting that the genetic pathway for tapetum development is generally conserved between rice and Arabidopsis
10-1298/N
ISSN:1001-6538
1861-9541