Molecular cloning and functional analysis of the FLOWERING LOCUS T （FT） homolog GhFT1 from Gossypium hirsutum
FLOWERING LOCUS T （FT） encodes a member of the phosphatidylethanolamine-binding protein （PEBP） family that functions as the mobile floral signal, playing an important role in regulating the floral transition in angiosperms. We isolated an FT-homolog （GhFT1） from Gossypium hirsutum L. cultivar, Xinlu...
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| Published in | 植物学报：英文版 no. 6; pp. 522 - 533 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
2015
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| Subjects | |
| Online Access | Get full text |
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| Summary: | FLOWERING LOCUS T （FT） encodes a member of the phosphatidylethanolamine-binding protein （PEBP） family that functions as the mobile floral signal, playing an important role in regulating the floral transition in angiosperms. We isolated an FT-homolog （GhFT1） from Gossypium hirsutum L. cultivar, Xinluzao 33 GhFT1 was predominantly expressed in stamens and sepals, and had a relatively higher expression level during the initiation stage of fiber development. GhFT1 mRNA displayed diurnal oscillations in both long-day and short-day condition, suggesting that the expression of this gene may be under the control of the circadian clock. Subcel ular analysis revealed that GhFT1 protein located in the cytoplasm and nucleus. Ectopic expression of GhFT1 in transgenic arabidopsis plants resulted in early flowering compared with wild-type plants. In addition, ectopic expression of GhFT1 in arabidopsis ft-10 mutants partial y rescued the extremely late flowering phenotype. Finally, several＆amp;nbsp;flowering related genes functioning downstream of AtFT were highly upregulated in the 35S：：GhFT1 transgenic arabidopsis plants. In summary, GhFT1 is an FT-homologous gene in cotton that regulates flower transition similar to its orthologs in other plant species and thus it may be a candidate target for promoting early maturation in cotton breeding. |
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| Bibliography: | FLOWERING LOCUS T （FT） encodes a member of the phosphatidylethanolamine-binding protein （PEBP） family that functions as the mobile floral signal, playing an important role in regulating the floral transition in angiosperms. We isolated an FT-homolog （GhFT1） from Gossypium hirsutum L. cultivar, Xinluzao 33 GhFT1 was predominantly expressed in stamens and sepals, and had a relatively higher expression level during the initiation stage of fiber development. GhFT1 mRNA displayed diurnal oscillations in both long-day and short-day condition, suggesting that the expression of this gene may be under the control of the circadian clock. Subcel ular analysis revealed that GhFT1 protein located in the cytoplasm and nucleus. Ectopic expression of GhFT1 in transgenic arabidopsis plants resulted in early flowering compared with wild-type plants. In addition, ectopic expression of GhFT1 in arabidopsis ft-10 mutants partial y rescued the extremely late flowering phenotype. Finally, several＆amp;nbsp;flowering related genes functioning downstream of AtFT were highly upregulated in the 35S：：GhFT1 transgenic arabidopsis plants. In summary, GhFT1 is an FT-homologous gene in cotton that regulates flower transition similar to its orthologs in other plant species and thus it may be a candidate target for promoting early maturation in cotton breeding. Danli Guo, Chao Li, Rui Dong, Xiaobo Li, Xiangwen Xiao and Xianzhong Huang（1Key Laboratory of Agrobiotechnology, College of Life Sciences, Shihezi University, Shihezi 832003, China, 2Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, the Chinese Academy of Sciences, Ururnqi 830011, China） Cotton; floral transition; florigen; flowering time; FLOWING LOCUS T 11-5067/Q |
| ISSN: | 1672-9072 1744-7909 |