Characterization, tissue distribution, and expression of neuropeptide Yin olive flounder Paralichthys olivaceus

Neuropeptide Y （NPY） is a 36-amino acid peptide of the neuropeptide Y family that plays key roles in the regulation of food intake. In this study, we focused on NPY mRNA expression changes around feeding time and during food deprivation in olive flounder. The olive flounder NPY mRNA levels were anal...

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Published in中国海洋湖沼学报：英文版 no. 3; pp. 553 - 558
Main Author 王倩 谭训刚 杜少军 孙威 尤锋 张培军
Format Journal Article
LanguageEnglish
Published 2015
Subjects
mRNA水平
mRNA表达
NPY
牙鲆
神经肽Y
组织分布
表征
食物摄入量
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ISSN0254-4059
1993-5005

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Summary:Neuropeptide Y （NPY） is a 36-amino acid peptide of the neuropeptide Y family that plays key roles in the regulation of food intake. In this study, we focused on NPY mRNA expression changes around feeding time and during food deprivation in olive flounder. The olive flounder NPY mRNA levels were analyzed in different tissues and a high level of expression was detected in the brain. We also demonstrated a correlation between NPY expression levels in the brain and feeding schedule. NPY expression levels in olive flounder maintained on a daily scheduled feeding regimen increased shortly before feeding and decreased after the scheduled feeding time. Compared with the -1 h group before feeding, NPY expression in the 3 h group after feeding decreased significantly （P〈0.05）. Food deprivation led to an 81.7% decrease in NPY mRNA levels in the 24 h fasted group （P〈0.05） and a 91.7% decrease in the 48 h fasted group （P〈0.05）. Therefore, our study demonstrates that NPY expression is associated with food intake in olive flounder. This result reveals the function of NPY in regulating food intake and its potential importance in olive flounder aquaculture.
Bibliography:WANG Qian , TAN Xungang , DU Shaojun , SUN Wei , YOU Feng , ZHANG Peijun （1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 2 University of Chinese Academy of Sciences, Beijing 100049, China 3Department of Biochemistry and Molecular Biology, School of Medicine, University of Maryland 701 E. Pratt St, Baltimore MD, 21202, USA）
37-1150/P
Neuropeptide Y （NPY） is a 36-amino acid peptide of the neuropeptide Y family that plays key roles in the regulation of food intake. In this study, we focused on NPY mRNA expression changes around feeding time and during food deprivation in olive flounder. The olive flounder NPY mRNA levels were analyzed in different tissues and a high level of expression was detected in the brain. We also demonstrated a correlation between NPY expression levels in the brain and feeding schedule. NPY expression levels in olive flounder maintained on a daily scheduled feeding regimen increased shortly before feeding and decreased after the scheduled feeding time. Compared with the -1 h group before feeding, NPY expression in the 3 h group after feeding decreased significantly （P〈0.05）. Food deprivation led to an 81.7% decrease in NPY mRNA levels in the 24 h fasted group （P〈0.05） and a 91.7% decrease in the 48 h fasted group （P〈0.05）. Therefore, our study demonstrates that NPY expression is associated with food intake in olive flounder. This result reveals the function of NPY in regulating food intake and its potential importance in olive flounder aquaculture.
neuropeptide Y; brain; fasting; olive flounder; feeding behavior; Paralichthys olivaceus
ISSN:0254-4059
1993-5005