Molecular characterization and expression analysis of a novel dual-CRD C-type lectin in kuruma shrimp(Marsupenaeus japonicus)
C-type lectins are among the most significant pattern recognition receptors(PRRs) found in invertebrate. They are a class of carbohydrate-binding proteins that can recognize specific sugar moieties on the surface of pathogens. In the present study, a novel C-type lecitn(termed Mj Lectin) from kuruma...
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Published in | 海洋学报:英文版 Vol. 34; no. 2; pp. 74 - 83 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
2015
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Subjects | |
Online Access | Get full text |
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Summary: | C-type lectins are among the most significant pattern recognition receptors(PRRs) found in invertebrate. They are a class of carbohydrate-binding proteins that can recognize specific sugar moieties on the surface of pathogens. In the present study, a novel C-type lecitn(termed Mj Lectin) from kuruma shrimp Marsupenaeus japonicus was identified. The full-length c DNA of Mj Lectin was 1 245 bp with a 1 011 bp open reading frame(ORF) that encoded a polypeptide of 336 amino acid residues. Mj Lectin consisted of two tandemly arrayed carbohydrate-recognition domains(CRDs), unlike other reported M. japonicus C-type lectins with only one CRD. It showed a high similarity to other shrimp dual-CRD lectins. Among the Ca2+-binding Site 2, the tripeptide motif dictating the carbohydrate binding specificity was exhibited as a rare mutant LPN(Leu134-Pro135-Asn136) in CRD1 and a traditional EPN(Glu299-Pro300-Asn301) in CRD2, respectively. Mj Lectin showed a specific expression pattern in both tissue and cellular levels, for its m RNA transcript was mainly expressed in the F-cells of the hepatopancreas. After white spot syndrome virus(WSSV) challenge(3.6×108 virions/μL), the expression of Mj Lectin in the hepatopancreas was up-regulated significantly at 48 h(P〈0.01) compared with the control group. These results suggested that Mj Lectin might be involved in the innate immune defense against WSSV infection. |
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Bibliography: | 11-2056/P C-type lectins are among the most significant pattern recognition receptors(PRRs) found in invertebrate. They are a class of carbohydrate-binding proteins that can recognize specific sugar moieties on the surface of pathogens. In the present study, a novel C-type lecitn(termed Mj Lectin) from kuruma shrimp Marsupenaeus japonicus was identified. The full-length c DNA of Mj Lectin was 1 245 bp with a 1 011 bp open reading frame(ORF) that encoded a polypeptide of 336 amino acid residues. Mj Lectin consisted of two tandemly arrayed carbohydrate-recognition domains(CRDs), unlike other reported M. japonicus C-type lectins with only one CRD. It showed a high similarity to other shrimp dual-CRD lectins. Among the Ca2+-binding Site 2, the tripeptide motif dictating the carbohydrate binding specificity was exhibited as a rare mutant LPN(Leu134-Pro135-Asn136) in CRD1 and a traditional EPN(Glu299-Pro300-Asn301) in CRD2, respectively. Mj Lectin showed a specific expression pattern in both tissue and cellular levels, for its m RNA transcript was mainly expressed in the F-cells of the hepatopancreas. After white spot syndrome virus(WSSV) challenge(3.6×108 virions/μL), the expression of Mj Lectin in the hepatopancreas was up-regulated significantly at 48 h(P〈0.01) compared with the control group. These results suggested that Mj Lectin might be involved in the innate immune defense against WSSV infection. C-type lectin,Marsupenaeus japonicus,carbohydrate-recognition domain(CRD),gene expression,white spot syndrome virus(WSSV) |
ISSN: | 0253-505X 1869-1099 |