γ链细胞因子对慢性乙型肝炎患者CD8^＋T细胞上TIM-3表达的调节

目的通过检测T细胞免疫球蛋白黏蛋白分子（TIM-3）在慢性乙型肝炎（CHB）患者外周血单个核细胞（PBMC）CD8^＋T细胞上的表达,探讨共用γ链细胞因子对CHB患者CD8^＋T细胞上TIM-3表达的影响。方法选取2014年1-5月在第四军医大学唐都医院传染科就诊的CHB初治患者15例,以及健康体检者8例。抽取两组全血,利用Ficoll密度梯度离心法分离出PBMC。分别给予γ链细胞因子白细胞介素（IL）2、IL-7、IL-15、IL-21和人抗CD3/CD28刺激,未刺激孔作为阴性对照。培养4 d后,用单克隆抗体染色,采用流式细胞仪检测CD8^＋T细胞上TIM-3的表达情况。计量资料比较采用成...

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Published in临床肝胆病杂志 Vol. 31; no. 2; pp. 205 - 208
Main Author 董杰 杨晓飞 申焕君 赵荣荣 赵燕燕 张野 黄长形 连建奇
Format Journal Article
LanguageChinese
Published 2015
Subjects
CD8阳性T淋巴细胞
乙型
免疫球蛋白类
慢性
白细胞介素类
肝炎
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ISSN1001-5256

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Summary:目的通过检测T细胞免疫球蛋白黏蛋白分子（TIM-3）在慢性乙型肝炎（CHB）患者外周血单个核细胞（PBMC）CD8^＋T细胞上的表达,探讨共用γ链细胞因子对CHB患者CD8^＋T细胞上TIM-3表达的影响。方法选取2014年1-5月在第四军医大学唐都医院传染科就诊的CHB初治患者15例,以及健康体检者8例。抽取两组全血,利用Ficoll密度梯度离心法分离出PBMC。分别给予γ链细胞因子白细胞介素（IL）2、IL-7、IL-15、IL-21和人抗CD3/CD28刺激,未刺激孔作为阴性对照。培养4 d后,用单克隆抗体染色,采用流式细胞仪检测CD8^＋T细胞上TIM-3的表达情况。计量资料比较采用成组t检验。结果与未刺激组相比,TIM-3在CD8^＋T细胞上表达如下：人抗CD3/CD28和IL-2、IL-15、IL-7刺激组显著升高,分别为（9.629±9.916）%,P=0.000 1;（3.817±2.694）%,P=0.000 6;（5.772±4.732）%,P=0.005 4;（3.560±2.045）%,P=0.030 2。IL-21刺激组表达虽有所增加,为（2.503±2.117）%,但差异无统计学意义,P=0.934 1。结论人抗CD3/CD28、γ链细胞因子中的IL-2、IL-7和IL-15都能够有效上调TIM-3在CHB患者CD8＋T细胞上的表达,提示通过抑制它们不仅可以下调TIM-3的表达,而且可能会增强CHB患者体内CD8＋T细胞的杀伤作用。
Bibliography:DONG Jie, YANG Xiaofei, SHEN Huanjun, et al. （Department of lnfectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi＇an 710038, China）
hepatitis B, chronic ; interleukins ; immunoglobulins ; CD8 - positive T - lymphocytes
Objective To measure the expression of T - cell immunoglobulin - and mucin domain - 3 - containing molecule 3 （ TIM - 3 ） on CD8 ^＋ T cells in peripheral blood mononuclear cells （PBMCs） among patients with chronic hepatitis B （ CHB ） and to investigate the effect of common gamma - chain cytokines on the expression of TIM - 3 on CD8^ ＋ T ceils in these patients. Methods Fifteen previously un- treated patients with CHB who visited the Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, from Janu- ary to May, 2014, as well as 8 healthy controls, were included in the study. Blood was collected from these subjects, and PBMCs were isolated from blood by Ficoll density gradient centrifugation. PBMCs were separately stimulated with gamma - chain
ISSN:1001-5256