木薯亲本正反交亲和力与授粉柱头的蛋白质组学分析

为研究木薯不同亲本杂交亲和力的差异,分别以华南5号、华南6号、华南7号为亲本进行正反交,统计不同组合间稔实率的差异。同时对受精柱头蛋白质进行分析,研究柱头对杂交亲和力影响的蛋白质调控水平。结果表明：SC5（♀）×SC7（♂）平均稔实率最高为26.22%,SC7（♀）×SC5（♂）平均稔实率最低为11.13%。通过双向电泳和质谱法分析母本SC5和SC7授粉柱头的蛋白质变化,得到27个差异蛋白质点,其中19个蛋白质出现上调表达,这些差异表达蛋白质分别参与碳代谢及能量代谢、分子伴侣和氨基酸代谢;而8个蛋白质下调表达,它们分别参与蛋白质合成及氢氰酸代谢;表明这些代谢途径对木薯亲和力高低有一定的影响。...

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Published in植物遗传资源学报 Vol. 16; no. 2; pp. 264 - 268
Main Author 叶剑秋 安飞飞 肖鑫辉 王明 许瑞丽 万仲卿 李开绵
Format Journal Article
LanguageChinese
Published 2015
Subjects
木薯
正反交
稔实率
蛋白质组
Online AccessGet full text
ISSN1672-1810

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Summary:为研究木薯不同亲本杂交亲和力的差异,分别以华南5号、华南6号、华南7号为亲本进行正反交,统计不同组合间稔实率的差异。同时对受精柱头蛋白质进行分析,研究柱头对杂交亲和力影响的蛋白质调控水平。结果表明：SC5（♀）×SC7（♂）平均稔实率最高为26.22%,SC7（♀）×SC5（♂）平均稔实率最低为11.13%。通过双向电泳和质谱法分析母本SC5和SC7授粉柱头的蛋白质变化,得到27个差异蛋白质点,其中19个蛋白质出现上调表达,这些差异表达蛋白质分别参与碳代谢及能量代谢、分子伴侣和氨基酸代谢;而8个蛋白质下调表达,它们分别参与蛋白质合成及氢氰酸代谢;表明这些代谢途径对木薯亲和力高低有一定的影响。
Bibliography:YE Jian-qiu, AN Fei-fei, XIAO Xin-hui, WANG Ming, XU Rui-li, WAN Zhong-qing, LI Kai-mian （ 1 College of Agriculture, Hainan university, Haikou 570228 ; 2 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Ministry of Agriculture for Germplasm Resources Conservation and Utilization of Cassava, Danzhou Hainan 571737）
In order to study the affinity differences of hybrid cassava between different parents,respectively,South China 5,South China 6 and South China 7 were as the materials,to conduct reciprocal cross,then fertility rates was calculated between different combinations. The proteins from stigma after fertilization were also analyzed,due to exploring the regulation mechanisms of stigma influence on hybridization affinity. The results showed that： fertility rates in SC5（ ♀） × SC7（ ♂） was highest,26. 22%,while SC7（ ♀） × SC5（ ♂） was lowest,11. 13%. By two-dimensional electrophoresis and mass spectrometry analysis of pollinated stigma protein changes
ISSN:1672-1810