王浆主蛋白（MRJPs）对张氏肝细胞的促增殖作用及其机制

将王浆主蛋白（major royal jelly proteins,MRJPs）添加到细胞培养基中,用MTT法比较纯MRJPs与胎牛血清（fetal bovine serum,FBS）、不同MRJPs/FBS比例对张氏肝细胞增殖的影响,用流式细胞仪检测MRJPs各处理对细胞周期的影响。结果表明：仅添加MRJPs不能促进细胞增殖;完全培养基（10%FBS）中MRJPs的最佳添加量为0.5mg/mL;MRJPs（5mg/mL）/FBS的添加比例为60/40时混合培养基对细胞的促增殖效果与完全培养基相比差异无统计学意义（P〉0.05）。流式细胞仪检测结果显示,MRJPs与FBS配合使用可促使细胞S期...

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Bibliographic Details
Published in浙江大学学报：农业与生命科学版 Vol. 41; no. 1; pp. 7 - 14
Main Author 于张颖 谌迪 王一然 沈立荣
Format Journal Article
LanguageChinese
Published 2015
Subjects
促增殖
张氏肝细胞
替代
王浆主蛋白
细胞周期
胎牛血清
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ISSN1008-9209

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Summary:将王浆主蛋白（major royal jelly proteins,MRJPs）添加到细胞培养基中,用MTT法比较纯MRJPs与胎牛血清（fetal bovine serum,FBS）、不同MRJPs/FBS比例对张氏肝细胞增殖的影响,用流式细胞仪检测MRJPs各处理对细胞周期的影响。结果表明：仅添加MRJPs不能促进细胞增殖;完全培养基（10%FBS）中MRJPs的最佳添加量为0.5mg/mL;MRJPs（5mg/mL）/FBS的添加比例为60/40时混合培养基对细胞的促增殖效果与完全培养基相比差异无统计学意义（P〉0.05）。流式细胞仪检测结果显示,MRJPs与FBS配合使用可促使细胞S期及G0/G1期比例增大;推测MRJPs的作用可能与促进DNA合成、前期RNA和核糖体合成有关。
Bibliography:major royal jelly proteins （MRJPs）; Chang＇s liver cell line; fetal bovine serum （FBS） ; proliferation;substitution; cell cycle
Summary Major royal jelly proteins （MRJPs） are the soluble proteins in royal jelly, which account for 82%- 90% of total royal jelly proteins. MRJPs are consisted of ten members, i.e. MRJP1 - MRJP9 and MRJPq2, whose biological functions include enhancing cell proliferation, inducing differentiation, modulating immune responses, accelerating wound healing, etc. MRJP1 with 57 ku in molecular mass was found possessing proliferation-promoting activities, enhancing proliferation of primary cultured rat hepatocytes and increasing albumin production in the absence of serum. MRJPs could significantly stimulate the proliferation and growth of endothelial progenitor cells （EPC）. The recombinant MRJP1 could significantly stimulate growth of Tn-5B-4 cell from Trichoplusia hi, and affect cell shape and adhesion to the substrate. Similar activities were also found in normal human neonatal skin fibrob
ISSN:1008-9209