Developing transgenic maize （Zea mays L.） with insect resistance and glyphosate tolerance by fusion gene transformation

• Published in: 农业科学学报：英文版 no. 2; pp. 305 - 313
• Main Author: SUN He LANG Zhi-hong LU Wei ZHANG Jie HE Kang-lai ZHU Li LIN Min HUANG Da-fang
• Format: Journal Article
• Language: English
• Published: 2015
• Subjects: EPSPS基因; 单子叶植物; 基因转化系统; 抗草甘膦; 抗虫; 根癌农杆菌介导; 苏云金芽孢杆菌; 转基因玉米
• ISSN: 2095-3119; 2352-3425

Using linker peptide LP4/2A for multiple gene transformation is considered to be an effective method to stack or pyramid several traits in plants. Bacillus thuringiensis（Bt） cry gene and epsps（5-enolpyruvylshikimate-3-phosphate synthase） gene are two important genes for culturing pest-resistant and glyphosate-tolerant crops. We used linker peptide LP4/2A to connect the Bt cry1 Ah gene with the 2m G2-epsps gene and combined the wide-used man A gene as a selective marker to construct one coordinated expression vector called p2 EPUHLAGN. The expression vector was transferred into maize by Agrobacterium tumefaciens-mediated transformation, and 60 plants were obtained, 40% of which were positive transformants. Molecular detection demonstrated that the two genes in the fusion vector were expressed simultaneously and spliced correctly in translation processing; meanwhile bioassay detection proved the transgenic maize had preferable pest resistance and glyphosate tolerance. Therefore, linker peptide LP4/2A provided a simple and reliable strategy for producing gene stacking in maize and the result showed that the fusion gene transformation system of LP4/2A was feasible in monocot plants.