铜陵重金属富集的尾矿废弃地酸性废水中细菌及古菌的多样性

为了加深对安徽省铜陵市不同重金属富集程度的尾矿附近酸性废水中微生物多样性的了解,运用PCR-16S r DNA克隆文库技术对酸性废水中细菌及古菌的群落结构进行研究。在铜陵的5个样点采集了8个水样。系统发育分析结果表明,酸性废水中的细菌主要是Betaproteobacteria,Gammaproteobacteria,Alphaproteobacteria,Deinococcus-Thermus,Nitrospira,Firmicutes,Actinobacteria,Deltaproteobacteria,Bacteroidetes,Chloroflexi,古菌是Thermoplasma,Fe...

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Published in中国有色金属学报：英文版 Vol. 24; no. 10; pp. 3332 - 3342
Main Author 杨扬 李杨 孙庆业
Format Journal Article
LanguageEnglish
Published 2014
Subjects
克隆文库
微生物结构组成
理化因子
酸性矿业废水
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Summary:为了加深对安徽省铜陵市不同重金属富集程度的尾矿附近酸性废水中微生物多样性的了解,运用PCR-16S r DNA克隆文库技术对酸性废水中细菌及古菌的群落结构进行研究。在铜陵的5个样点采集了8个水样。系统发育分析结果表明,酸性废水中的细菌主要是Betaproteobacteria,Gammaproteobacteria,Alphaproteobacteria,Deinococcus-Thermus,Nitrospira,Firmicutes,Actinobacteria,Deltaproteobacteria,Bacteroidetes,Chloroflexi,古菌是Thermoplasma,Ferroplasma以及Thermogymnomonas。根据细菌和古菌的组成对样品进行聚类分析,结果表明,5个样品由于优势菌株Thermomonas和Meiothermus拥有较高的相似度。2个样品由于Acidithiobacillus和Leptospirillum占主导也拥有较高的相似度。剩余的样品多样性较高,拥有最高的香浓-微纳指数2.91。典型相关分析CCA的结果表明微生物的多样性组成与理化因子有密切的关系,如水的p H以及Hg2＋、Pb2＋、Cl-、SO2-4、Fe3＋的含量。
Bibliography:43-1239/TG
Yang YANG,Yang LI,Qing-ye SUN（School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China）
To expand knowledge on microbial communities of various metal-rich levels of mine drainage environments in Anhui province, China, the archaeal and bacterial diversities were examined using a PCR-based cloning approach. Eight acid mine water samples were collected from five areas in Tongling. Phylogenetic analyses revealed that bacteria mainly fell into ten divisions, which were Betaproteobacteria, Gammaproteobacteria, Alphaproteobacteria, Deinococcus-Thermus, Nitrospira, Firmicutes, Actinobacteria, Deltaproteobacteria, Bacteroidetes, Chloroflexi. Archaea fell into three phylogenetic divisions, Thermoplasma, Ferroplasma and Thermogymnomonas. The unweighted pair group method with arithmetic mean（UPGMA） cluster analysis based on the microbial communities’ compositions revealed that five samples shared similarity with the dominance of Meiothermus and Thermomonas. Two samples had the preponderant existence of Acidithiobacillus and Leptospirillum. The remaining sample owned higher microbial communities’ diversity with the Shannon-Weaver H up to 2.91. Canonical correlation analysis（CCA） suggested that microbial community structures had great association with p H and the concentration of Hg2＋, Pb2＋, Fe3＋, Cl-, SO2- 4in water.
acid mine drainage;microbial community;clone library;geochemical variables
ISSN:1003-6326