胶质瘤干细胞与神经干细胞基因表达差异的微阵列基因芯片分析

目的利用基因芯片技术筛选人脑胶质瘤干细胞（GSCs）和正常神经干细胞（NSCs）中差异表达的基因。方法利用人类HOA5.1 OneArray表达谱芯片（包括29 186个基因）,与GSCs和NSCs的总RNA生成的探针进行杂交,通过寡核苷酸芯片ScanArray 4000筛选两者之间的差异基因,并选择其中的部分差异表达基因（DCX、PTGS2、SCGN、GAD2、OTX2、PEG10、NRXN3）进一步行qRT-PCR验证。结果与正常NSCs相比,GSCs中1372个基因表达下调,1501个基因表达上调,功能富集分析显示,这些差异基因主要参与了神经轴突导向、细胞周期、细胞黏附、免疫炎症反应及癌...

Full description

Saved in:
Bibliographic Details
Published in解放军医学杂志 Vol. 39; no. 10; pp. 800 - 803
Main Author 尹丰 张剑宁 赵明明 周春辉 王淑为 郭欣如 刘爽
Format Journal Article
LanguageChinese
Published 2014
Subjects
寡核苷酸序列分析
神经干细胞
神经胶质瘤
肿瘤干细胞
Online AccessGet full text

Cover

More Information
Summary:目的利用基因芯片技术筛选人脑胶质瘤干细胞（GSCs）和正常神经干细胞（NSCs）中差异表达的基因。方法利用人类HOA5.1 OneArray表达谱芯片（包括29 186个基因）,与GSCs和NSCs的总RNA生成的探针进行杂交,通过寡核苷酸芯片ScanArray 4000筛选两者之间的差异基因,并选择其中的部分差异表达基因（DCX、PTGS2、SCGN、GAD2、OTX2、PEG10、NRXN3）进一步行qRT-PCR验证。结果与正常NSCs相比,GSCs中1372个基因表达下调,1501个基因表达上调,功能富集分析显示,这些差异基因主要参与了神经轴突导向、细胞周期、细胞黏附、免疫炎症反应及癌症相关的信号路径。qRT-PCR检测结果与芯片检测结果相符。结论多类基因在胶质瘤的发生发展中发挥着重要作用,该结果可为胶质瘤的靶向治疗提供新的线索。
Bibliography:glioma; tumor stem cells; neural stem cells; oligonucleotide array sequence analysis
Objective To identify the differential expressed genes of human glioma stem cells（GSCs） and human neural stem cells（NSCs） by gene chip technology. Methods Human HOA5.1 OneArray microarray（including 29 186 genes） was adopted and hybridized with probes which were prepared from the total RNAs of GSCs and NSCs. Differential expressed genes between the GSCs and NSCs were assayed after scanning oligonucleotide microarray with ScanArray 4000, and some of these genes such as DCX, PTGS2, SCGN, GAD2, OTX2, PEG10 and NRXN3 were verified by real-time-Q-PCR method. Results Compared with the genes in normal NSCs, 1372 down-regulated and 1501 up-regulated genes in GSCs were revealed by means of microarray, and these genes were associated with axon guidance, cell cycle, cell adhesion, immune-inflammatory responses and cancer-related signal pathways. The results of qRT-PCR were consistent with that of microarray. Conclusions Multiple genes pla
ISSN:0577-7402