萎叶提取物增强5-氟尿嘧啶对结肠癌细胞HT29和HCT116的生长抑制作用

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Bibliographic Details
Published in浙江大学学报：B卷英文版 Vol. 15; no. 8; pp. 692 - 700
Main Author Pek Leng NG Nor Fadilah RAJAB Sue Mian THEN Yasmin Anum MOHD YUSOF Wan Zurinah WAN NGAH Kar Yong PIN Mee Lee LOOI
Format Journal Article
LanguageEnglish
Published 2014
Subjects
5-氟尿嘧啶
等效线图法
胡椒科
草药-药物相互作用
蒌叶
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Bibliography:Piperaceae, Piper beUe L., 5-Fiuorouracil, Isobologram analysis, Herb-drug interaction
Objective： The combination effect of Piperbetle （PB） and 5-fluorouracil （5-FU） in enhancing the cytotoxic potential of 5-FU in inhibiting the growth of colon cancer cells was investigated. Methods： HT29 and HCT116 cells were subjected to 5-FU or PB treatment. 5-FU and PB were then combined and their effects on both cell lines were observed after 24 h of treatment. PB-5-FU interaction was elucidated by isobologram analysis. Apoptosis features of the treated cells were revealed by annexin V/PI stain. High-performance liquid chromatography （HPLC） was performed to exclude any possible chemical interaction between the compounds. Results： In the presence of PB extract, the cytotoxicity of 5-FU was observed at a lower dose （IC~0 12.5 pmol/L） and a shorter time （24 h） in both cell lines. Both cell lines treated with 5-FU or PB alone induced a greater apoptosis effect compared with the combination treatment. Isobologram analysis indicated that PB and 5-FU interacted synergistically and antagonistically in inhibiting the growth of HT29 and HCT116 cells, respectively. Conclusions： In the presence of PB, a lower dosage of 5-FU is required to achieve the maximum drug effect in inhibiting the growth of HT29 cells. However, PB did not significantly reduce 5-FU dosage in HCT116 cells. Our result showed that this interaction may not solely contribute to the apoptosis pathway.
33-1356/Q
ISSN:1673-1581
1862-1783