Plasma microRNA profiling in nasopharyngeal carcinoma patients reveals miR-548q and miR-483-5p as potentia lbiomarkers

MicroRNAs （miRNAs）, which play a role in tumorigenesis, may also serve as diagnostic or prognostic biomarkers. However, studies on human miRNA profiles in plasma from nasopharyngeal carcinoma （NPC） patients are in their infancy. Here, we used microarrays to perform systematic profiling of human miRN...

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Published in癌症：英文版 Vol. 33; no. 7; pp. 330 - 338
Main Author Xiao-Hui Zheng Cui Cui Hong-Lian Ruan Wen-Qiong Xue Shao-Dan Zhang Ye-Zhu Hu Xin-Xi Zhou Wei-Hua Jia
Format Journal Article
LanguageEnglish
Published 2014
Subjects
microRNA
miRNA
全国人民代表大会
患者
生物标志物
聚合酶链反应
血浆
鼻咽癌
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Summary:MicroRNAs （miRNAs）, which play a role in tumorigenesis, may also serve as diagnostic or prognostic biomarkers. However, studies on human miRNA profiles in plasma from nasopharyngeal carcinoma （NPC） patients are in their infancy. Here, we used microarrays to perform systematic profiling of human miRNAs in plasma from NPC patients. We subsequently used realtime quantitative polymerase chain reaction （Q-PCR） to validate miRNAs with aberrant expression that could serve as potential biomarkers. By comparing the plasma miRNA profiles of 31 NPC patients and 19 controls, 39 of 887 human miRNAs were found to be aberrantly expressed. Considering the fold change and P value, miR-548q and miR-483- 5p were validated in 132 samples from 82 NPC patients and 50 controls. Moreover, high expression of miR-548q and miR483-5p was further found in 3 NPC cell lines and clinical biopsy tissues from 54 NPC patients and 22 controls. Our results revealed that miR-548q and miR-483-5p are potential biomarkers of NPC. Combining the receiver operating characteristic （ROC） analyses of these 2 miRNAs, an area under the ROC curve （AUC） of 0.737 with 67.1% sensitivity and 68.0% specificity were obtained, showing the preliminary diagnostic value of plasma miRNAs. Moreover, most NPC patients with a poor outcome exhibited high expression （〉 median） of miR548q （70.6%） and miR-483-5p （64.7%） in tissue samples, indicating their prognostic value. The high expression levels of miR-548q and miR-483-5p in plasma, cell lines, and clinical tissues of NPC patients indicate that their roles in NPC should be explored in the future.
Bibliography:Nasopharyngeal carcinoma （NPC）;microRNA profiles;potential biomarkers
MicroRNAs （miRNAs）, which play a role in tumorigenesis, may also serve as diagnostic or prognostic biomarkers. However, studies on human miRNA profiles in plasma from nasopharyngeal carcinoma （NPC） patients are in their infancy. Here, we used microarrays to perform systematic profiling of human miRNAs in plasma from NPC patients. We subsequently used realtime quantitative polymerase chain reaction （Q-PCR） to validate miRNAs with aberrant expression that could serve as potential biomarkers. By comparing the plasma miRNA profiles of 31 NPC patients and 19 controls, 39 of 887 human miRNAs were found to be aberrantly expressed. Considering the fold change and P value, miR-548q and miR-483- 5p were validated in 132 samples from 82 NPC patients and 50 controls. Moreover, high expression of miR-548q and miR483-5p was further found in 3 NPC cell lines and clinical biopsy tissues from 54 NPC patients and 22 controls. Our results revealed that miR-548q and miR-483-5p are potential biomarkers of NPC. Combining the receiver operating characteristic （ROC） analyses of these 2 miRNAs, an area under the ROC curve （AUC） of 0.737 with 67.1% sensitivity and 68.0% specificity were obtained, showing the preliminary diagnostic value of plasma miRNAs. Moreover, most NPC patients with a poor outcome exhibited high expression （〉 median） of miR548q （70.6%） and miR-483-5p （64.7%） in tissue samples, indicating their prognostic value. The high expression levels of miR-548q and miR-483-5p in plasma, cell lines, and clinical tissues of NPC patients indicate that their roles in NPC should be explored in the future.
44-1195/R
ISSN:1000-467X
1944-446X