Role of Matrix Metalloproteinase- 1 （MMP- 1）/Protease-activated Receptor-1 （PAR-1）Signaling Pathway in the Cervical Cancer Invasion

Objective To investigate the role of matrix metalloproteinase-1 （MMP-1）/protease- activated receptor-1 （PAR-l） signaling in the cervical cancer invasion. Methods RT-PCR was used to test the mRNA level of MMP-1 in 59 cases of cervical squamous cell cancer. Cell invasion was evaluated by Transwell ass...

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Published in生殖与避孕：英文版 no. 1; pp. 18 - 25
Main Author Ning-xia SUN Qian ZHAO Chen YE Yan MA Wen LI
Format Journal Article
LanguageEnglish
Published 2014
Subjects
Hela细胞
MMP-1
RT-PCR方法
信令
入侵途径
基质金属蛋白酶
宫颈癌
蛋白酶激活受体
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Summary:Objective To investigate the role of matrix metalloproteinase-1 （MMP-1）/protease- activated receptor-1 （PAR-l） signaling in the cervical cancer invasion. Methods RT-PCR was used to test the mRNA level of MMP-1 in 59 cases of cervical squamous cell cancer. Cell invasion was evaluated by Transwell assay to explore the effect of adding human recombinant MMP-1 （rhMMP-1） and PARI-siRNA on cervical cancer invasion. Results In cervical cancer tissues, more MMP-1 expression was observed than that in the normal cervical tissues, and its expression correlated with cancer status. Human recombinant MMP-1 （rhMMP-1） could promote Hela cell invasion, and its number of invasive cell correlated with the concentration of rhMMP-1. Disrupting the expression of PAR-1 reduced the MMP-1 promoting-effect on Hela cell invasion, but had no effect on non-MMP-1 invasive action. Conclusion The MMP-1/PAR-1 signaling pathway is involved in cervical cancer invasion. Therefore, blocking PAR-1 may represent a new therapeutic option for metastatic cervical cancer.
Bibliography:Objective To investigate the role of matrix metalloproteinase-1 （MMP-1）/protease- activated receptor-1 （PAR-l） signaling in the cervical cancer invasion. Methods RT-PCR was used to test the mRNA level of MMP-1 in 59 cases of cervical squamous cell cancer. Cell invasion was evaluated by Transwell assay to explore the effect of adding human recombinant MMP-1 （rhMMP-1） and PARI-siRNA on cervical cancer invasion. Results In cervical cancer tissues, more MMP-1 expression was observed than that in the normal cervical tissues, and its expression correlated with cancer status. Human recombinant MMP-1 （rhMMP-1） could promote Hela cell invasion, and its number of invasive cell correlated with the concentration of rhMMP-1. Disrupting the expression of PAR-1 reduced the MMP-1 promoting-effect on Hela cell invasion, but had no effect on non-MMP-1 invasive action. Conclusion The MMP-1/PAR-1 signaling pathway is involved in cervical cancer invasion. Therefore, blocking PAR-1 may represent a new therapeutic option for metastatic cervical cancer.
31-1555/R
cervical cancer; matrix metalloproteinase-1 （MMP-1）; protease-activatedreceptor-1 （PAR-l）; metastasis; invasion
ISSN:1001-7844