一种高效的柑橘绿霉菌基因敲除体系的构建

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Bibliographic Details
Published in浙江大学学报：B卷英文版 Vol. 15; no. 2; pp. 116 - 124
Main Author Qian xU Cong-yi ZHU Ming-shang WANG Xue-peng SUN Hong-ye LI
Format Journal Article
LanguageEnglish
Published 2014
Subjects
Ku80
基因敲除
指状青霉
非同源末端链接（NHEJ）
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Bibliography:Penicillium digitatum, Efficiency, Gene targeting, Non-homologous end-joining （NHEJ） pathway, Ku80
Penicillium digitatum is the most important pathogen of postharvest citrus. Gene targeting can be done in P. digitatum using homologous recombination via Agrobacterium tumefaciens mediated transformation （ATMT）, but the frequencies are often very low. In the present study, we replaced the Ku80 homolog （a gene of the non-homologous end-joining （NHEJ） pathway） with the hygromycin resistance cassette （hph） by ATMT. No significant change in vegetative growth, conidiation, or pathogenicity was observed in KuSO-deficient strain （△dKuSO） of P. digitatum. However, using △pdKuSO as a targeting strain, the gene-targeting frequencies for both genes PdbrlA and PdmpkA were significantly increased. These results suggest that Ku80 plays an important role in homologous inte- gration and the created △PdKuSO strain would be a good candidate for rapid gene function analysis in P. digitatum.
33-1356/Q
ISSN:1673-1581
1862-1783