沉默BRG1基因对人胶质瘤细胞增殖的影响及其机制

目的探讨小干扰RNA（siRNA）沉默胶质瘤细胞BRG1基因表达对其增殖的影响及其机制。方法化学合成BRGlsiRNA，脂质体介导转染胶质瘤U251和U87细胞。CCK-8细胞增殖实验检测细胞增殖，流式细胞仪分析细胞周期变化，Westernblot检测BRGl、cyclin家族、CDK抑制剂蛋白表达水平。结果转染BRGlsiRNA可以有效减少胶质瘤U251和U87N胞BRG1表达，细胞增殖下降，G期细胞增加。BRG1沉默后cyclinD1和cyclinB1表达降低，CDK抑制剂p21和p27没有明显变化。结论沉默胶质瘤细胞BRG1表达影响细胞周期蛋白使细胞停滞在G1期，最终抑制胶质瘤细胞增殖。...

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Published in肿瘤防治研究 Vol. 40; no. 12; pp. 1134 - 1137
Main Author 时梅林 梅鹏金 范月超 李中林 白津 徐凯 郑骏年
Format Journal Article
LanguageChinese
Published 2013
Subjects
BRGl
增殖
细胞周期
胶质瘤
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Summary:目的探讨小干扰RNA（siRNA）沉默胶质瘤细胞BRG1基因表达对其增殖的影响及其机制。方法化学合成BRGlsiRNA，脂质体介导转染胶质瘤U251和U87细胞。CCK-8细胞增殖实验检测细胞增殖，流式细胞仪分析细胞周期变化，Westernblot检测BRGl、cyclin家族、CDK抑制剂蛋白表达水平。结果转染BRGlsiRNA可以有效减少胶质瘤U251和U87N胞BRG1表达，细胞增殖下降，G期细胞增加。BRG1沉默后cyclinD1和cyclinB1表达降低，CDK抑制剂p21和p27没有明显变化。结论沉默胶质瘤细胞BRG1表达影响细胞周期蛋白使细胞停滞在G1期，最终抑制胶质瘤细胞增殖。
Bibliography:42-1241/R
BRG 1 ;Glioma;Proliferation;Cell cycle
SHI Meilin, MEI Pengjin, FAN Yuechao, LI Zhonglin, BAI Jin, XU Kai, ZHENG Junnian（ 1. Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou 221002, China;2. School of Medical Imaging, Xuzhou Medical College;3. Department of Neurosurgery, The Affiliated Hospital of Xuzhou Medical College）
Objective To investigate the effect of siRNA silencing BRG1 gene on glioma cell proliferation and its molecular mechanism. Methods Chemically synthesized small interfering RNA targeting BRG1 was transfected into human glioma cell lines U251 and U87 by siLentFect Lipid Reagent. The proliferation of both glioma cells after BRG1 interference was measured by CCK-8 assay. The cell cycles of glioma cells were detected by flow cytometry.The expression levels of BRG1, cyclin family and CDK inhibitor proteins were detected by western blot. Results BRG1 interference could drastically decrease the ability of cell proliferation in both glioma cells. The GI popula
ISSN:1000-8578