DNA methylation polymorphism in flue-cured tobacco and candidate markers for tobacco mosaic virus resistance
DNA methylation plays an important role in the epigenctic regulation of gent expression during plant growth, development, and polyploidization. However, there is still no distinct evidence in tobacco regarding the distribution of the methylation pattern and whether it contributes to qualitative char...
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| Published in | 浙江大学学报：B卷英文版 Vol. 14; no. 7; p. 577 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
2013
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| Subjects | |
| Online Access | Get full text |
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| Summary: | DNA methylation plays an important role in the epigenctic regulation of gent expression during plant growth, development, and polyploidization. However, there is still no distinct evidence in tobacco regarding the distribution of the methylation pattern and whether it contributes to qualitative characteristics. We studied the levels and patterns of methylation polymorphism at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco, Nicotiana tabacum, using a methylation-sensitive amplified polymorphism （MSAP） technique. The results showed that rncthylation existed at a high level among tobacco accessions, among which 49.3% sites were methylated and 69.9% allelic sites wcrc polymorphic. A cluster analysis revealed distinct patterns of geography-specific groups. In addition, three polymorphic sites significantly related to tobacco mosaic virus （TMV） resistance were explored, This suggests that tobacco breeders should pay more attention to epigcnetic traits. |
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| Bibliography: | DNA methylation plays an important role in the epigenctic regulation of gent expression during plant growth, development, and polyploidization. However, there is still no distinct evidence in tobacco regarding the distribution of the methylation pattern and whether it contributes to qualitative characteristics. We studied the levels and patterns of methylation polymorphism at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco, Nicotiana tabacum, using a methylation-sensitive amplified polymorphism （MSAP） technique. The results showed that rncthylation existed at a high level among tobacco accessions, among which 49.3% sites were methylated and 69.9% allelic sites wcrc polymorphic. A cluster analysis revealed distinct patterns of geography-specific groups. In addition, three polymorphic sites significantly related to tobacco mosaic virus （TMV） resistance were explored, This suggests that tobacco breeders should pay more attention to epigcnetic traits. 33-1356/Q |
| ISSN: | 1673-1581 1862-1783 |