Novel nanometer scaffolds regulate the biologica behaviors of neural stem cells

• Published in: 中国神经再生研究：英文版 Vol. 8; no. 16; pp. 1455 - 1464
• Main Author: Jihui Zhou Fuge Sui Meng Yao Yansong Wang Yugang Liu Feipeng Tian Qiang Li Xiaofeng He Lin Shao Zhiqiang Liu
• Format: Journal Article
• Language: English
• Published: 2013
• Subjects: caspase-3; 支架材料; 新型纳米纤维; 生物防治; 神经干细胞; 细胞周期蛋白D1; 细胞周期蛋白依赖性激酶; 行为
• ISSN: 1673-5374

Ideal tissue-engineered scaffold materials regulate proliferation, apoptosis and differentiation of cells seeded on them by regulating gene expression. In this study, aligned and randomly oriented collagen nanofiber scaffolds were prepared using electronic spinning technology. Their diameters and appearance reached the standards of tissue-engineered nanometer scaffolds. The nanofiber scaffolds were characterized by a high swelling ratio, high porosity and good mechanical properties. The proliferation of spinal cord-derived neural stem cells on novel nanofiber scaffolds was obviously enhanced. The proportions of cells in the S and G2/M phases noticeably increased. Moreover, the proliferation rate of neural stem cells on the aligned collagen nanofiber scaffolds was high. The expression levels of cyclin D1 and cyclin-dependent kinase 2 were increased. Bcl-2 expression was significantly increased, but Bax and caspase-3 gene expressions were obviously decreased. There was no significant difference in the differentiation of neural stem cells into neurons on aligned and randomly oriented collagen nanofiber scaffolds. These results indicate that novel nanofiber scaffolds could promote the proliferation of spinal cord-derived neural stem cells and inhibit apoptosis without inducing differentiation. Nanofiber scaffolds regulate apoptosis and proliferation in neural stem cells by altering gene expression.