Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria

Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both eco- logical and biological significance. This st...

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Bibliographic Details
Published in中国科学：技术科学英文版 no. 3; pp. 766 - 776
Main Author FU WanTao ZHANG JuLin ZHENG ChangBo LIU Jing AN ZhongFu LIU HongWen ZHANG Wei
Format Journal Article
LanguageEnglish
Published 2013
Subjects
14-3-3蛋白
mRNA表达
分子克隆
基因
常见细菌
感染性
海洋生态系统
海绵
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Summary:Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both eco- logical and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Re- verse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water--E, coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was ex- posed to E. coli （1.1×10^7 CFU mL^-1）, V. anguillarum II （1.2×10^6 CFU mL^-1） and V. alginolyticus （3.6×10^5 CFU mL-1） for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was ob- served on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The re- sults demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.
Bibliography:marine sponge Hymeniacidon perleve, 14-3-3 mRNA, RT-PCR, infectious bacteria
Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both eco- logical and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Re- verse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water--E, coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was ex- posed to E. coli （1.1×10^7 CFU mL^-1）, V. anguillarum II （1.2×10^6 CFU mL^-1） and V. alginolyticus （3.6×10^5 CFU mL-1） for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was ob- served on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The re- sults demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.
11-5845/TH
ISSN:1674-7321
1869-1900