Changes in secretory pathway Ca2＋ATPase 2 following focal cerebral ischemia/reperfusioninjury

• Published in: 中国神经再生研究：英文版 Vol. 8; no. 1; pp. 76 - 82
• Main Author: Tonglin Lu Zhiping Hu Liuwang Zeng Zheng Jiang
• Format: Journal Article
• Language: English
• Published: 2013
• Subjects: mRNA表达水平; 再灌注损伤; 分泌途径; 大脑皮质; 大鼠脑; 缺血; 蛋白质; 逆转录PCR; 钙ATP酶
• ISSN: 1673-5374

This study aimed to investigate changes in secretory pathway Ca2＋-ATPase 2 expression following cerebral ischemia/reperfusion injury, and to define the role of Ca2＋-ATPases in oxidative stress. A rat model of cerebral ischemia/reperfusion injury was established using the unilateral middle cerebral artery occlusion method. Immunohistochemistry and reverse transcription-PCR assay results showed that compared with the control group, the expression of secretory pathway Ca2＋-ATPase 2 protein and mRNA in the cerebral cortex and hippocampus of male rats did not significantly change during the ischemic period. However, secretory pathway Ca2＋-ATPase 2 protein and mRNA expression reduced gradually at 1, 3, and 24 hours during the reperfusion period. Our experimental findings indicate that levels of secretory pathway Ca2＋-ATPase 2 protein and mRNA expression in brain tissue change in response to cerebral ischemia/reperfusion injury.