Changes in secretory pathway Ca2＋ATPase 2 following focal cerebral ischemia/reperfusioninjury

This study aimed to investigate changes in secretory pathway Ca2＋-ATPase 2 expression following cerebral ischemia/reperfusion injury, and to define the role of Ca2＋-ATPases in oxidative stress. A rat model of cerebral ischemia/reperfusion injury was established using the unilateral middle cerebral a...

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Published in中国神经再生研究：英文版 Vol. 8; no. 1; pp. 76 - 82
Main Author Tonglin Lu Zhiping Hu Liuwang Zeng Zheng Jiang
Format Journal Article
LanguageEnglish
Published 2013
Subjects
mRNA表达水平
再灌注损伤
分泌途径
大脑皮质
大鼠脑
缺血
蛋白质
逆转录PCR
钙ATP酶
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Summary:This study aimed to investigate changes in secretory pathway Ca2＋-ATPase 2 expression following cerebral ischemia/reperfusion injury, and to define the role of Ca2＋-ATPases in oxidative stress. A rat model of cerebral ischemia/reperfusion injury was established using the unilateral middle cerebral artery occlusion method. Immunohistochemistry and reverse transcription-PCR assay results showed that compared with the control group, the expression of secretory pathway Ca2＋-ATPase 2 protein and mRNA in the cerebral cortex and hippocampus of male rats did not significantly change during the ischemic period. However, secretory pathway Ca2＋-ATPase 2 protein and mRNA expression reduced gradually at 1, 3, and 24 hours during the reperfusion period. Our experimental findings indicate that levels of secretory pathway Ca2＋-ATPase 2 protein and mRNA expression in brain tissue change in response to cerebral ischemia/reperfusion injury.
Bibliography:neural regeneration; brain injury; cerebral infarction; secretory pathway Ca2＋-ATPase 2; Golgiapparatus; Ca2＋ oscillations; manganese; focal cerebral ischemia; oxidative damage; Ca2~-ATPase;grants-supported paper; photographs-containing paper; neuroregeneration
Tonglin Lu, Zhiping Hu, Liuwang Zeng, Zheng Jiang（ Department of Neurology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China）
This study aimed to investigate changes in secretory pathway Ca2＋-ATPase 2 expression following cerebral ischemia/reperfusion injury, and to define the role of Ca2＋-ATPases in oxidative stress. A rat model of cerebral ischemia/reperfusion injury was established using the unilateral middle cerebral artery occlusion method. Immunohistochemistry and reverse transcription-PCR assay results showed that compared with the control group, the expression of secretory pathway Ca2＋-ATPase 2 protein and mRNA in the cerebral cortex and hippocampus of male rats did not significantly change during the ischemic period. However, secretory pathway Ca2＋-ATPase 2 protein and mRNA expression reduced gradually at 1, 3, and 24 hours during the reperfusion period. Our experimental findings indicate that levels of secretory pathway Ca2＋-ATPase 2 protein and mRNA expression in brain tissue change in response to cerebral ischemia/reperfusion injury.
11-5422/R
ISSN:1673-5374