Detection of K88 Fimbriae Gene of Escherichia coil from Mink

[Objective] This paper aimed to study the mechanism of diarrhea of mink caused by Escherichia coil [Method] Through the detection of K88 fimbriae gene of E. coli, cloning of gene fragments and identification, then PCR amplification was used to detect adhesion factor K88 gene, which was connected to...

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Bibliographic Details
Published in动物与饲料科学：英文版 Vol. 4; no. 5; pp. 206 - 208
Main Author ZHANG Yan-ying GAO Gui-sheng GAO Guang-ping SHI Qiu-mei
Format Journal Article
LanguageEnglish
Published 2012
Subjects
K88菌毛
PCR扩增
基因检测
大肠杆菌K88
感受态细胞
水貂
菌毛基因
阳性克隆
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Summary:[Objective] This paper aimed to study the mechanism of diarrhea of mink caused by Escherichia coil [Method] Through the detection of K88 fimbriae gene of E. coli, cloning of gene fragments and identification, then PCR amplification was used to detect adhesion factor K88 gene, which was connected to T-vector and transformed into competent cells, and positive clones were selected. [ Results] E. coli 078, 029 and 038 were isolated from organs and feces of mink died of diarrhea in 3 mink farms, respectively, the 3 serotypes of E. coliwere detected in carrying K88 fimbriae gene and 3 positive clones were screened, respectively. [ Conclusion] The E. coli causing mink diarrhea carry K88 fimbriae gene.
Bibliography:Mink; Diarrhea; K88 fimbriae gene; Clone
[Objective] This paper aimed to study the mechanism of diarrhea of mink caused by Escherichia coil [Method] Through the detection of K88 fimbriae gene of E. coli, cloning of gene fragments and identification, then PCR amplification was used to detect adhesion factor K88 gene, which was connected to T-vector and transformed into competent cells, and positive clones were selected. [ Results] E. coli 078, 029 and 038 were isolated from organs and feces of mink died of diarrhea in 3 mink farms, respectively, the 3 serotypes of E. coliwere detected in carrying K88 fimbriae gene and 3 positive clones were screened, respectively. [ Conclusion] The E. coli causing mink diarrhea carry K88 fimbriae gene.
ISSN:1943-9911