Effect of Bushenyisui Formula on brain tissue apoptosis and Bcl-2 in beta-amyloid protein-induced Alzheimer＇s disease rat models

• Published in: 中医杂志：英文版 Vol. 32; no. 4; pp. 646 - 650
• Main Author: Shuke Cui Yan Sun Chengzang Liu
• Format: Journal Article
• Language: English
• Published: 2012
• Subjects: Bcl-2蛋白; β-淀粉样蛋白; 大鼠模型; 氯化钠溶液; 细胞凋亡; 脑组织; 补肾; 阿尔茨海默氏病
• ISSN: 0255-2922

OBJECTIVE： To investigate the effect of Bushenyisui Formula on cell apoptosis and positive B cell lym- phoma （Bcl-2） in the Brain of rat models of Alzheim- er＇s disease （AD） induced by beta-amyloid protein （All3） and the mechanism underlying the effect. METHODS： Total of 40 SD rats, 20 females and 20 males, were randomly assigned to 4 groups, con- trolled group （A）, model group （B）, conventional treatment group （C） and Bushenyisui Formula treat- ment （BYFT） group （D）, 10 rats in each group. AI3 1-42 was injected into the bilateral hippocampus of the rats in group B, C and D to create the models of AD. Sham operation was performed on the rats of group A in the same way by injecting equal volume of 0.9% sodium chloride solution into their bilateral hippocampus. 5 days after operation, Bushenyisui Formula was intraperitoneally administered at a dose of 450 mg/kg to the rats of group D （QD） for 20 days. Equal volume of 0.9% sodium chloride solution was intraperitoneally injected into the rats of group B with the same procedure. C suspension （20 mg/mL） was intraperitoneally injected into the rats of group B with the same procedure. The number of apoptot- ic cells in Brain and the positive Bcl-2 were count- ed. The changes of learning and memory abilities were evaluated using Y-maze. RESULTS： Right after the establishment of the mod- els, group B, C and D compared to group A respec- tively, the outcomes of Y-maze were significantly different from that of group A, which suggested ob- vious learning and memory disorder in those groups （P〈0.01）. After treatment, the times of elec- tronic shocks of group C and D were significantly less than that of group B （P〈0.05）, and the numbers of apoptotic cells and positive Bcl-2 were signifi- cantly different from those of group B, apoptotic sells＇ number of group C and D smaller than that of group B and the number of positive Bcl-2 greater than that of group B. CONCLUSION： Bushenyisui Formula could increase the number of Bcl-2 in brain, which improved the function of nervous system pertaining to learning and memory abilities.