Cloning and Sequence Analysis of Porcine IRGC Gene
[ Objective] This study was to analyze porcine IRGC gene, so as to lay the foundation of further revealing its function. [ Method] IRGC gene was cloned by using EST information together with sequencing, then aligned with the IRGC sequences of human, cattle, dog and orangutan for obtaining their simi...
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Published in | 动物与饲料科学:英文版 no. 6; pp. 10 - 11 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
2009
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Subjects | |
Online Access | Get full text |
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Summary: | [ Objective] This study was to analyze porcine IRGC gene, so as to lay the foundation of further revealing its function. [ Method] IRGC gene was cloned by using EST information together with sequencing, then aligned with the IRGC sequences of human, cattle, dog and orangutan for obtaining their similarities. [Result] Porcine IRGC cDNA obtained in the present study was 1 558 bp in length(GenBank accession number EU703776), which shared high similarity with human (86%), cattle (90%), dog (91%), orangutan (84%). Sequence analysis shows that porcine IRGC gene encodes 464 amino acid residues which share high homologies with the counterparts from human (90%), cattle (93%), dog (95%), orangutan (90%). [Condusion] Cloning and sequence analysis of porcine IRGC gene would be helpful for studying its functions. |
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Bibliography: | Pig; IRGC gene; Clone; Sequence analysis [ Objective] This study was to analyze porcine IRGC gene, so as to lay the foundation of further revealing its function. [ Method] IRGC gene was cloned by using EST information together with sequencing, then aligned with the IRGC sequences of human, cattle, dog and orangutan for obtaining their similarities. [Result] Porcine IRGC cDNA obtained in the present study was 1 558 bp in length(GenBank accession number EU703776), which shared high similarity with human (86%), cattle (90%), dog (91%), orangutan (84%). Sequence analysis shows that porcine IRGC gene encodes 464 amino acid residues which share high homologies with the counterparts from human (90%), cattle (93%), dog (95%), orangutan (90%). [Condusion] Cloning and sequence analysis of porcine IRGC gene would be helpful for studying its functions. |
ISSN: | 1943-9911 |