宫内发育迟缓对仔猪小肠基因表达的影响（摘要）

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Published in动物与饲料科学：英文版 no. 4; pp. 41 - 46
Main Author 陈荣军 高运苓 孔祥峰 潘杰 李铁军 印遇龙
Format Journal Article
LanguageEnglish
Published 2009
Subjects
仔猪
基因芯片
实时定量RT-PCR
宫内发育迟缓
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Bibliography:CHEN Rong-jun, GAO Yun-Iing, KONG Xiang-feng, PAN Jie, LI Tie-junI, YIN Yu-long（ 1. Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; 2. International Center of Future Agriculture for Human Health, Sichuan Agricultural University, Chengdu 611134, China; 3. Graduate University, Chinese Academy of Sciences, Beijing 100039, China）
Intrauterine growth restriction; Piglets; Microarray; Real-time quantitative PCR
[ Objective] To profile the differentially expressed genes in small intestine between piglets with intrauterine growth restriction （IUGR）, describe the relationships between growth performance and gene expression in IUGR piglets, and thus provide a theoretical basis for further research. [Metbed] Eight suckling piglets at the age of 21 d Efour with normal body weight （NBW） of （1 503 ± 310） g and four with low BW of （806 ±35） g] were killed, and the intestinal samples were collected. Gene expression was detected by Affymetrix Porcine GeneChip and further confirmed by quantitative real-time PCR. [ ReseltJ Microarray analysis showed that there were 156 differentially expressed genes in the small intestine between the IUGR piglets and the age-matched NBW piglets, including 61 down-regulated genes and 95 up-regulated genes, The up-regulated genes included protein tyrosine phosphatase, myosin, troponin, heat shock protein, metallothionein, arginine vasopressin-induced 1, ribosomal protein L6, apoptosls antagonizing transcription factor, muscle creatine kinase, mannosidase, lysozyme, folliculin, urate transporterchannel protein, pyrroline-5-carboxylate reductese-like, and adenine phosphor-dbosyltransferase. The down-regulated genes included protein kinase, arachidohate 12-1ipoxygenase, transcription factor A, GTP-GDP dissociation stimulator 1, serine （or cysteine） proteinase inhibitor, fetuin, dolichol-phosphate-mannose synthase, apolipoprotein H, argininosuccinate synthetase 1, iron-regulated transporter, alpha-2-macroglobulin, immunoglobulin superfamily, thioltransferase, and guanylate binding protein 2. The gene expression profile changed in the small intestine of piglets with intrauterine growth restriction, providing a theoretical basis for eady intervention in growth restriction.
ISSN:1943-9911