活细胞实时成像技术研究抗坏血酸（AA）协同砷剂抗人骨肉瘤MG-63的体外疗效
目的研究抗坏血酸（ascorbic acid，AA）和三氧化二砷（arsenic trioxide，As2O3）抗入骨肉瘤细胞Mp63的体外疗效。方法以MG-63细胞为体外模型，用62．5μmol／LAA与1μmol／LAs2O3单独或联合处理细胞，利用新型连续活细胞图像采集和分析平台（continuous live cell imaging and analysis platform，Cell IQ）实时观察细胞的生长情况和形态学的变化。结果1μmol／LAs2O3和62．5μmol／LAA单独处理都可抑制MG-63细胞的生长并诱导细胞死亡。1μmol／LAs2O3和62．5μmot／LAA...
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| Published in | 复旦学报：医学版 Vol. 39; no. 6; pp. 649 - 652 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
2012
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| Subjects | |
| Online Access | Get full text |
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| Summary: | 目的研究抗坏血酸（ascorbic acid，AA）和三氧化二砷（arsenic trioxide，As2O3）抗入骨肉瘤细胞Mp63的体外疗效。方法以MG-63细胞为体外模型，用62．5μmol／LAA与1μmol／LAs2O3单独或联合处理细胞，利用新型连续活细胞图像采集和分析平台（continuous live cell imaging and analysis platform，Cell IQ）实时观察细胞的生长情况和形态学的变化。结果1μmol／LAs2O3和62．5μmol／LAA单独处理都可抑制MG-63细胞的生长并诱导细胞死亡。1μmol／LAs2O3和62．5μmot／LAA联合处理细胞较单独处理组细胞抑制效果更明显。结论AA和AS2O3抗人骨肉瘤细胞Mg-63可起到协同作用，这一结果为临床治疗骨肉瘤提供了新的思路和实验依据。 |
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| Bibliography: | Objective To study the synergistic effect of human osteosarcoma cell line MG-63 treated with ascorbic acid （AA） and arsenic trioxide （As2O3 ） in vitro. Methods We used continuous live cell imaging and analysis platform （cell IQ） to observe cell proliferation and morphologie change of MG-63 cells which were treated with AA （62.5 μmol/L） and As203 （1 μmol/L） alone or together . Results MCr-63 cell proliferation was depressed observed when treated by AA （62. 5 μmol/L） and As203 （1μmol/L） independently. The effect of AA （62. 5 μmol/L） plus As2 03 （ 1 μmol/L） was synergistic, which further inhibits MG-63 cell proliferation. Conclusions The treatment of AA combined with As2O3 can induce synergistic effect on the depression of MG-63 cell proliferation. This result provides a new pathway and basic reaserch data of treating osteosarcoma in clinical practice. HUANG Xiao-chun , LI Ze-bing , CHEN Zeng-gan , CHEN Tong-yi , WANG Ling yan （ 1. Department of Rehabilitation; 2 Department of orthopedic; 3 .Biomedical Research C |
| ISSN: | 1672-8467 |