职业锰暴露人群外周血铁代谢的变化观察

目的探讨职业锰暴露对作业工人外周血铁代谢的影响。方法采用个体空气采样器采集空气锰,确定锰的时间加权平均暴露剂量（TWA）,按照不同暴露剂量将工人分为暴露组（n=85,TWA〉0.2mg/m3）和对照组（n=80,TWA〈0.05mg/m3）。采用火焰原子吸收仪和石墨炉原子吸收仪测定工人血浆、血细胞中锰和铁的含量,ELISA法等检测两组血清铁蛋白、转铁蛋白、转铁蛋白受体、总铁结合力的浓度。结果暴露组血浆锰、血细胞锰含量均高于对照组（P〈0.01）。与对照组工人比较,工龄〈5年的暴露组工人血浆铁、血细胞铁、血清转铁蛋白受体、血清总铁结合力均降低（P〈0.01）,而血清转铁蛋白升高（P〈0.01）,...

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Published in解放军医学杂志 Vol. 37; no. 11; pp. 1069 - 1072
Main Author 熊云刚 申旭波 姜慧 贾飞飞 范奇元 邹焰
Format Journal Article
LanguageChinese
Published 2012
Subjects
职业暴露
铁代谢障碍
锰
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Summary:目的探讨职业锰暴露对作业工人外周血铁代谢的影响。方法采用个体空气采样器采集空气锰,确定锰的时间加权平均暴露剂量（TWA）,按照不同暴露剂量将工人分为暴露组（n=85,TWA〉0.2mg/m3）和对照组（n=80,TWA〈0.05mg/m3）。采用火焰原子吸收仪和石墨炉原子吸收仪测定工人血浆、血细胞中锰和铁的含量,ELISA法等检测两组血清铁蛋白、转铁蛋白、转铁蛋白受体、总铁结合力的浓度。结果暴露组血浆锰、血细胞锰含量均高于对照组（P〈0.01）。与对照组工人比较,工龄〈5年的暴露组工人血浆铁、血细胞铁、血清转铁蛋白受体、血清总铁结合力均降低（P〈0.01）,而血清转铁蛋白升高（P〈0.01）,血清铁蛋白升高（P〈0.05）,工龄≥5年的暴露组工人血浆铁降低（P〈0.05）,血清转铁蛋白升高（P〈0.01）,血清转铁蛋白受体降低（P〈0.01）。与工龄〈5年的暴露组工人比较,工龄≥5年的暴露组工人血细胞锰降低（P〈0.05）,血细胞铁升高（P〈0.05）,其余指标差异无统计学意义（P〉0.05）。暴露组工人工龄与血浆锰、血细胞锰、血浆铁、血细胞铁、血清铁蛋白、转铁蛋白、转铁蛋白受体、总铁结合力等指标之间无线性相关性（P〉0.05）。结论长期锰暴露可致外周血铁代谢失衡,具体表现为血浆铁、血清转铁蛋白受体降低及血清转铁蛋白升高。
Bibliography:Objectives To investigate the adverse effect of manganese exposure on the iron metabolism in peripheral blood of professionally exposed workers. Methods The manganese in air was collected using personal air sampler, and the time weighted average （TWA） concentration of exposure to manganese was then calculated. The subjects were divided into exposure group （n=85） and control group （n=80） based on the exposure doses they received. The concentrations of iron and manganese in the plasma and blood cells of the subjects were determined using flame atomic absorption detector and graphite furnace atomic absorption detector. Serum ferritin, transferrin, transferrin receptor and total iron binding capacity were determined using enzyme linked immunosorbent assay. Results The manganese contents in both plasma and blood cells were much higher in exposure group than in control group （P〈0.01）. Compared with the control group, the iron contents in both plasma and blood cells, serum transferrin receptor and total iron binding
ISSN:0577-7402