Overexpression of Rice Sphingosine-l-Phoshpate Lyase Gene OsSPL1 in Transgenic Tobacco Reduces Salt and Oxidative Stress Tolerance

Sphingolipids, including sphingosine-l-phosphate （SIP）, have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed functional analysis of a rice （Oryza sativa） SlP lyase gene OsSPL1 in transgenic tobac...

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Published in植物学报：英文版 Vol. 54; no. 9; pp. 652 - 662
Main Author Huijuan Zhang Jing Zhai Jibo Mo Dayong Li Fengming Song
Format Journal Article
LanguageEnglish
Published 2012
Subjects
低盐
氧化应激
水稻
耐受性
转基因烟草植株
过表达
酶基因
鞘氨醇
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Summary:Sphingolipids, including sphingosine-l-phosphate （SIP）, have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed functional analysis of a rice （Oryza sativa） SlP lyase gene OsSPL1 in transgenic tobacco plants and explored its possible involvement in abiotic stress response. Overexpression of OsSPL1 in transgenic tobacco resulted in enhanced sensitivity to exogenous abscisic acid （ABA）, and decreased tolerance to salt and oxidative stress, when compared with the wild type. Furthermore, the expression levels of some selected stress-related genes in OsSPLl-overexpressing plants were reduced after application of salt or oxidative stress, indicating that the altered responsiveness of stress-related genes may be responsible for the reduced tolerance in OsSPLl.overexpressing tobacco plants under salt and oxidative stress. Our results suggest that rice OsSPL1 plays an important role in abiotic stress responses.
Bibliography:11-5067/Q
Sphingolipids, including sphingosine-l-phosphate （SIP）, have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed functional analysis of a rice （Oryza sativa） SlP lyase gene OsSPL1 in transgenic tobacco plants and explored its possible involvement in abiotic stress response. Overexpression of OsSPL1 in transgenic tobacco resulted in enhanced sensitivity to exogenous abscisic acid （ABA）, and decreased tolerance to salt and oxidative stress, when compared with the wild type. Furthermore, the expression levels of some selected stress-related genes in OsSPLl-overexpressing plants were reduced after application of salt or oxidative stress, indicating that the altered responsiveness of stress-related genes may be responsible for the reduced tolerance in OsSPLl.overexpressing tobacco plants under salt and oxidative stress. Our results suggest that rice OsSPL1 plays an important role in abiotic stress responses.
Sphingolipids; sphingosine-l-phosphate lyase （SPL）; OsSPL1; salt stress; oxidative stress.
ISSN:1672-9072
1744-7909